IGEM:IMPERIAL/2007/DNA Constructs: Difference between revisions
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|colspan="3" align="center"| The assemblies displayed above will be used to test the properties of the promoters used in our devices. Both constructs use fluorescence as their reporter. | |colspan="3" align="center"| The assemblies displayed above will be used to test the properties of the promoters used in our devices. Both constructs use fluorescence as their reporter. | ||
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===Parts Description=== | |||
====[BBa_I0500]==== |
Revision as of 04:05, 6 August 2007
Construct Overview
Parts from the registry
A | W | S | Name | Description | Plasmid | Resist | Copy | Size | Stock | Project | Model |
A | W | BBa_E0040 | GFP | pSB1A2 | AmpR | High | 720bp | Well 5H, Plate1 | Shared Part | ||
A | W | BBa_I13522 | GFP under tetracyclin promoter | pSB1A2 | AmpR | High | 937bp | Well 15H, Plate2 | Basic Construct | ||
A | BBa_J5528 | GFP under arabinose promoter | pSB2K3 | KanR | Low | 2093bp | Well 6H, Plate3 | CBD | |||
A | W | BBa_J24677 | GFP under lux promoter, with luxR | pSB1A2 | AmpR | High | 1787bp | Well 18K, Plate3 | Basic Construct | ||
A | W | BBa_R0040 | tetracyclin promoter | pSB1A2 | AmpR | High | 54bp | Well 7O, Plate1 | Shared Part | ||
A | W | BBa_I0500 | arabinose promoter | pSB2K3 | KanR | Low | 1210bp | Well 9I, Plate2 | CBD | ||
A | W | BBa_F2620 | lux promoter, with luxR | pSB1A2 | AmpR | High | 1061bp | Well 6B, Plate1 | BF | ||
A | W | BBa_F1610 | luxI coding region | pSB1AK3 | A+KR | High | 798bp | Well 1B, Plate2 | BF | ||
A | W | BBa_R0010 | lac promoter | pSB1A2 | AmpR | High | 200bp | Well 7K, Plate1 | Hrp | ||
Our Parts
A | W | S | Name | Description | Plasmid | Resist | Copy | Size | Stock | Project | Model |
Cell By Date
- Construct CBD 1
- pT7 is currently in Biobricks Registry: BBa_J34814 (planning)
- pT7 can be used with T7 RNAP in vitro and in veso, and it gives constitutive expression.
- Construct CBD 2
- Entire construct is currently in Biobricks Registry: BBa_I13522 (available), in pSB1A2 (AmpR)
- pTet can be used with E.coli RNAP in vitro and maybe in veso, and it gives constitutive expression.
- Construct CBD 3
- Entire construct is currently in Biobricks Registry: BBa_J5528 (available), in pSB2K3 (KanR)
- Similar to part J5527 which has mRFP in place of GFP.
- pBad can be used with E.coli RNAP in vitro, and it is inducible by arabinose.
- To be used in veso, arabinose transport proteins have to be inserted in the phospholipid bilayer of the vesicles.
Biofilm Detector
- Construct BD 1
- Construct BD 2
- Entire part is currently in Biobricks Registry: BBa_J24677 (available), in pSB1A2 (AmpR)
- Entire part minus GFP reporter system is currently in Biobricks Registry: BBa_F2620 (available), in pSB1A2 (AmpR)
- GFP reporter system is currently in Biobricks Registry: BBa_I13504 (available)
- pLux can be used with E.coli RNAP in vitro and maybe in veso, and it is repressed by LuxR but activated by AHL:LuxR complex.
- Construct BD 3
- Entire part minus Hrp system is currently in Biobricks Registry: BBa_F2620 (available)
- pHrpL can be used with E.coli RNAP in vitro and maybe in veso, and it is inducible by HrpR:HrpS:Sigma 54 complex.
Maybe, you meant LuxR instead of LuxI. Also, with the Hrp system, try to have a generic and modular approach when you define your constructs. PoPs_IN -> PoPs_OUT
Hrp Constructs
Promoter A is pLux BBa_R0062 |
Two Test constructs will be made with different promoters, one with pBAD I0500 and one with pLac BBa_R0010 |
The assemblies displayed above will be used to test the properties of the promoters used in our devices. Both constructs use acGFP as their reporter. |
Promoter A is BBa_R0062 | A completed assembly of our first Hrp device that has only one input. We chose to add promoter (promoter A) and a fluorescence reporter (acGFP) as to make it functional and thus test it. A review of the actual device without the promoter\reporter can be found here . |
Part 1 | Promoter B will be either BBa_I0500 or BBa_R0010 | This includes promoter B which expresses HrpV and dsRFP at the same time. |
Part 2 | Promoter A is BBa_R0062 | This includes promoter A which expresses HrpR and HrpS eventually activating HrpL.Notice that this part is exactly the same as Hrp device 1 shown above. |
The assemblies displayed above will be used to test the properties of the promoters used in our devices. Both constructs use fluorescence as their reporter. |