IGEM:IMPERIAL/2007/DNA Constructs: Difference between revisions
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|| A || W || || || [http://parts.mit.edu/registry/index.php/Part:BBa_T9002 BBa_T9002] || GFP under lux promoter, with luxR || [http://parts.mit.edu/registry/index.php/Part:pSB1A3 pSB1A3] || AmpR || High || 1945bp || 4102bp || Well 19B, Plate 2 | || A || W || || || [http://parts.mit.edu/registry/index.php/Part:BBa_T9002 BBa_T9002] || GFP under lux promoter, with luxR || [http://parts.mit.edu/registry/index.php/Part:pSB1A3 pSB1A3] || AmpR || High || 1945bp || 4102bp || Well 19B, Plate 2 | ||
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|| A || W || || || [http://parts.mit.edu/registry/index.php/Part:BBa_B0034 BBa_B0034] || RBS || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 12bp || 2091bp || Well 3O, Plate 1 | |||
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Revision as of 03:57, 13 August 2007
Constructs Overview
Cloning Table
I | Part | Description | Status | Ligation Target | Use in Constructs |
BBa_E0040 | GFP | Minipreped | --- | Not being used anymore | |
BBa_I13522 | ptet-GFP | Minipreped | --- | Basic construct for chassis characterization | |
BBa_J5528 | pBad-GFP | Not Cloned | --- | Test construct for CBD (pBad) | |
I | BBa_J24677 | ptet-luxR-plux-GFP | Minipreped | --- | Not being used anymore |
BBa_R0040 | ptet | Minipreped | acGFP, dsRFP, luxI | CBD final (pBad), ID AHL sender, Hrp test construct | |
I | BBa_I0500 | pBad | Minipreped | acGFP, dsRFP | CBD final construct, Hrp test construct |
BBa_F2620 | ptet-luxR-plux | Minipreped | lacZ, Hrp amplifier | ID final construct | |
BBa_F1610 | luxI | Minipreped | ptet | ID AHL sender final construct | |
BBa_R0010 | lacI-pLac | Minipreped | --- | Not being used anymore | |
BBa_E1010 | RFP | Minipreped | --- | Not being used anymore | |
BBa_E7104 | T7 GFP | Minipreped | --- | CBD test construct (T7) | |
BBa_S03550 | RBS-AraC-noStop | Minipreped | ptet, pBad | CBD final construct (pBad) | |
BBa_B0015 | Double terminator | Minipreped | AraC | CBD test construct (pBad) | |
BBa_R0051 | λcI regulated promoter | Minipreped | acGFP, dsRFP | CBD final construct | |
BBa_J07037 | cI and GFP | Minipreped | --- | Not being used anymore | |
BBa_E0240 | GFP | Not cloned | cI | CBD test construct (cI) | |
BBa_I13507 | RFP | Not cloned | CBD promoters | CBD final reporter | |
BBa_T9002 | ptet-luxR-plux-GFP | Not cloned | --- | Basic construct for chassis characterization | |
BBa_B0034 | strong RBS | Not cloned | --- | Backup part | |
BBa_I719??? | T7 promoter | Pending | acGFP, dsRFP | CBD final construct | |
BBa_I719??? | acGFP | Ordered | ptet, pBad, plux | Reporter system for test constructs, Hrp | |
BBa_I719??? | dsRFP | Ordered | ptet, pBad, plux | Reporter system for ID, Hrp | |
BBa_I719??? | HrpR-HrpS-pHrpL | Ordered | Promoter, acGFP | Hrp core system | |
BBa_I719??? | HrpV | Ordered | Promoter, dsRFP | Hrp inhibitor system | |
BBa_I719??? | lacZ | Paused | plux, pHrp | ID final constructs |
Ligation Table
I | Part | Description | Status | Parents | Application |
BBa_I719??? | pcI-GFP | Pending | BBa_R0051 + GFP | CBD test construct | |
BBa_I719??? | pT7-dsRFP | Pending | pT7 + dsRFP | CBD construct | |
BBa_I719??? | ptet-dsRFP | Pending | BBa_R0040+ dsRFP | CBD final construct | |
BBa_I719??? | ptet-araC | Pending | BBa_R0040 + BBa_S03550 + BBa_B0015 | CBD final construct | |
BBa_I719??? | pBad-dsRFP | Pending | BBa_I0500 + dsRFP | CBD final construct (pBad) | |
BBa_I719??? | pcI-dsRFP | Pending | BBa_R0051 + dsRFP | CBD construct | |
BBa_I719??? | phrpL-GFP | Pending | pHrpL + GFP | ID AHL detector test construct | |
BBa_I719??? | ptet-luxI | Pending | R0040 + F1610 | ID AHL sender final construct | |
BBa_I719??? | ptet-luxR-Plux-X-gal | Pending | BBa_F2620 + lacZ | ID AHL detector final construct | |
BBa_I719??? | ptet-luxR-Plux-hrpRhrpS | Pending | BBa_F2620 + HrpRHrpS | ID AHL detector final construct | |
BBa_I719??? | phrpL-X-gal | Pending | pHrpL + lacZ | ID AHL detector final construct | |
Legend: I (Information)
Parts from the Registry
A | W | G | S | Name | Description | Plasmid | Resist | Copy | Size(B) | Size(T) | Location |
A | W | G | BBa_E0040 | GFP | pSB1A2 | AmpR | High | 720bp | 2799bp | Well 5H, Plate 1 | |
A | W | G | BBa_I13522 | GFP under tetracyclin promoter | pSB1A2 | AmpR | High | 937bp | 3016bp | Well 15H, Plate 2 | |
A | BBa_J5528 | GFP under arabinose promoter | pSB2K3 | KanR | Low | 2093bp | 6518bp | Well 6H, Plate 3 | |||
A | W | G | BBa_J24677 | GFP under lux promoter, with luxR | pSB1A2 | AmpR | High | 1787bp | 3866bp | Well 18K, Plate 3 | |
A | W | X | BBa_R0040 | tetracyclin promoter | pSB1A2 | AmpR | High | 54bp | 2133bp | Well 7O, Plate 1 | |
A | W | G | BBa_I0500 | arabinose promoter | pSB2K3 | KanR | Low | 1210bp | 5635bp | Well 9I, Plate 2 | |
A | W | G | BBa_F2620 | lux promoter, with luxR | pSB1A2 | AmpR | High | 1061bp | 3140bp | Well 6B, Plate 1 | |
A | W | G | BBa_F1610 | luxI coding region | pSB1AK3 | A+KR | High | 798bp | 3987bp | Well 1B, Plate 2 | |
A | W | G | BBa_R0010 | lac promoter | pSB1A2 | AmpR | High | 200bp | 2279bp | Well 7K, Plate 1 | |
A | G | BBa_E1010 | RFP | pSB2K3 | KanR | Low | 681bp | 5106bp | Well 15M, Plate 2 | ||
A | W | G | BBa_E7104 | T7 GFP | pSB1A2 | AmpR | High | 826bp | 2905bp | Well 13F, Plate 2 | |
A | G | BBa_S03550 | AraC with RBS but no stop | pSB1AC3 | A+CR | High | 958bp | 4013bp | Well 20E, Plate 3 | ||
A | W | G | BBa_B0015 | Double terminator | pSB1AK3 | A+KR | High | 129bp | 3316bp | Well 1B, Plate 2 | |
A | W | X | BBa_R0051 | λcI promoter | pSB1A2 | AmpR | High | 49bp | 2128bp | Well 9C, Plate 1 | |
A | G | BBa_J07037 | GFP under cI promoter | pSB2K3 | KanR | Low | 912bp | 5337bp | Well 12D, Plate 2 | ||
A | BBa_E0240 | GFP | pSB1A2 | AmpR | High | 876bp | 2955bp | Well 16A, Plate 1 | |||
A | BBa_I13507 | RFP | pSB1A2 | AmpR | High | 861bp | 2940bp | Well 16N, Plate 1 | |||
A | W | BBa_T9002 | GFP under lux promoter, with luxR | pSB1A3 | AmpR | High | 1945bp | 4102bp | Well 19B, Plate 2 | ||
A | W | BBa_B0034 | RBS | pSB1A2 | AmpR | High | 12bp | 2091bp | Well 3O, Plate 1 | ||
Legend: A (Available), W (Working), G (Checked on gel) [G - incorrect size; X - does not appear on gel], S (Sequenced)
Our Parts
A | W | G | S | Name | Description | Plasmid | Resist | Copy | Size(B) | Size(P) | Stock |
BBa_I791??? | luxI coding region under tetracyclin promoter | pSB1A2 | AmpR | High | 860bp | 2939bp | |||||
BBa_I791??? | dsRFP under tetracyclin promoter | pSB1A2 | AmpR | High | |||||||
BBa_I791??? | dsRFP under arabinose promoter | pSB2K3 | KanR | Low | |||||||
Constructs for Cell By Date
- Construct CBD 1
- Construct CBD 2
- Entire construct is currently in Biobricks Registry: BBa_I13522 (available), in pSB1A2 (AmpR)
- pTet can be used with E.coli RNAP in vitro and maybe in veso, and it gives constitutive expression.
- Construct CBD 3
- pBAD promoter with GFP is currently available in Biobricks Registry: BBa_J5528 (available), in pSB2K3 (KanR)
- Need Ara C regulator protein in vitro and in veso. Ara C available with the RBS:BBa_S03550
- pBad can be used with E.coli RNAP in vitro, and it is inducible by arabinose.
- To be used in veso, arabinose transport proteins have to be inserted in the phospholipid bilayer of the vesicles.
- Construct CBD 4
- Entire construct is currently available in Biobricks Registry: BBa_J07037 (avaiable), in pSB2K3 (KanR)
- pcI gives constitutive expression.
- Construct CBD 5
- Construct CBD 6
- Parts avaiable ....
- Construct CBD 7
- Parts avaiable ....
- Construct CBD 8
- Parts avaiable ....
- Construct CBD 9
- Parts avaiable ....
Constructs for Infector Detector
- Construct ID 1
- Construct ID 2
- Entire part is currently in Biobricks Registry: BBa_J24677 (available), in pSB1A2 (AmpR)
- Entire part minus GFP reporter system is currently in Biobricks Registry: BBa_F2620 (available), in pSB1A2 (AmpR)
- GFP reporter system is currently in Biobricks Registry: BBa_I13504 (available)
- pLux can be used with E.coli RNAP in vitro and maybe in veso, and it is repressed by LuxR but activated by AHL:LuxR complex.
- Construct ID 3