IGEM:IMPERIAL/2007/DNA Constructs: Difference between revisions

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|- style="background:#CCFFFF" align="center"
|width=10px|I
|width=10px|I
|width=20px|ID
|width=10%|Part
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|width=20%|Description
|width=20%|Description
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<!-- T7 PROMOTER -->
<!-- T7 PROMOTER -->
|- style="background:#CCFFFF" align="center"
|- style="background:#CCFFFF" align="center"
||  || [http://parts.mit.edu/registry/index.php?title=Part:BBa_E0040 BBa_E0040] || GFP || Minipreped || --- || ''Not being used anymore''
||  || 01 || [http://parts.mit.edu/registry/index.php?title=Part:BBa_E0040 BBa_E0040] || GFP || Minipreped || --- || ''Not being used anymore''
<!-- TETRACYCLIN PROMOTER WITH GFP-->
<!-- TETRACYCLIN PROMOTER WITH GFP-->
|- style="background:#CCFFFF" align="center"
|- style="background:#CCFFFF" align="center"
||  || [http://parts.mit.edu/registry/index.php/Part:BBa_I13522 BBa_I13522] || ptet-GFP || Minipreped || --- || Basic construct for chassis characterization
||  || 02 || [http://parts.mit.edu/registry/index.php/Part:BBa_I13522 BBa_I13522] || ptet-GFP || Minipreped || --- || Basic construct for chassis characterization


|- style="background:#CCFFFF" align="center"
|- style="background:#CCFFFF" align="center"
||  || [http://parts.mit.edu/registry/index.php/Part:BBa_J5528 BBa_J5528] || pBad-GFP || Not Cloned || --- || Test construct for CBD (pBad)
||  || 03 || [http://parts.mit.edu/registry/index.php/Part:BBa_J5528 BBa_J5528] || pBad-GFP || Cloning || --- || Test construct for CBD (pBad)


|- style="background:#CCFFFF" align="center"
|- style="background:#CCFFFF" align="center"
|| [[IGEM:IMPERIAL/2007/DNA_Constructs/BBa_J24677|I]] || [http://parts.mit.edu/registry/index.php/Part:BBa_J24677 BBa_J24677] || ptet-luxR-plux-GFP || Minipreped || --- || ''Not being used anymore''
|| [[IGEM:IMPERIAL/2007/DNA_Constructs/BBa_J24677|I]] || 04 || [http://parts.mit.edu/registry/index.php/Part:BBa_J24677 BBa_J24677] || ptet-luxR-plux-GFP || Minipreped || --- || ''Not being used anymore''


|- style="background:#CCFFFF" align="center"
|- style="background:#CCFFFF" align="center"
||  || [http://parts.mit.edu/registry/index.php/Part:BBa_R0040 BBa_R0040] || ptet || Minipreped || acGFP, DsRed, luxI || CBD final (pBad), ID AHL sender, Hrp test construct
||  || 05 || [http://parts.mit.edu/registry/index.php/Part:BBa_R0040 BBa_R0040] || ptet || Minipreped || acGFP, DsRed, luxI || CBD final (pBad), ID AHL sender, Hrp test construct


|- style="background:#CCFFFF" align="center"
|- style="background:#CCFFFF" align="center"
|| [[IGEM:IMPERIAL/2007/DNA_Constructs/BBa_I0500|I]] || [http://parts.mit.edu/registry/index.php/Part:BBa_I0500 BBa_I0500] || pBad || Minipreped || acGFP, DsRed || CBD final construct, Hrp test construct
|| [[IGEM:IMPERIAL/2007/DNA_Constructs/BBa_I0500|I]] || 06 ||[http://parts.mit.edu/registry/index.php/Part:BBa_I0500 BBa_I0500] || pBad || Minipreped || acGFP, DsRed || CBD final construct, Hrp test construct


|- style="background:#CCFFFF" align="center"
|- style="background:#CCFFFF" align="center"
||  || [http://parts.mit.edu/registry/index.php/Part:BBa_F2620 BBa_F2620] || ptet-luxR-plux || Minipreped || lacZ, Hrp amplifier || ID final construct
||  || 07 || [http://parts.mit.edu/registry/index.php/Part:BBa_F2620 BBa_F2620] || ptet-luxR-plux || Minipreped || lacZ, Hrp amplifier || ID final construct


|- style="background:#CCFFFF" align="center"
|- style="background:#CCFFFF" align="center"
||  || [http://parts.mit.edu/registry/index.php/Part:BBa_F1610 BBa_F1610] || luxI || Minipreped || ptet || ID AHL sender final construct
||  || 08 || [http://parts.mit.edu/registry/index.php/Part:BBa_F1610 BBa_F1610] || luxI || Minipreped || ptet || ID AHL sender final construct


|- style="background:#CCFFFF" align="center"
|- style="background:#CCFFFF" align="center"
||  || [http://parts.mit.edu/registry/index.php/Part:BBa_R0010 BBa_R0010] || lacI-pLac || Minipreped || --- || ''Not being used anymore''
||  || 09 || [http://parts.mit.edu/registry/index.php/Part:BBa_R0010 BBa_R0010] || lacI-pLac || Minipreped || --- || ''Not being used anymore''


|- style="background:#CCFFFF" align="center"
|- style="background:#CCFFFF" align="center"
||  || [http://parts.mit.edu/registry/index.php/Part:BBa_E1010 BBa_E1010] || RFP || Minipreped || --- || ''Not being used anymore''
||  || 10 || [http://parts.mit.edu/registry/index.php/Part:BBa_E1010 BBa_E1010] || RFP || Minipreped || --- || ''Not being used anymore''


|- style="background:#CCFFFF" align="center"
|- style="background:#CCFFFF" align="center"
||  || [http://parts.mit.edu/registry/index.php/Part:BBa_E7104 BBa_E7104] || T7 GFP || Minipreped || --- || CBD test construct (T7)
||  || 11 || [http://parts.mit.edu/registry/index.php/Part:BBa_E7104 BBa_E7104] || T7 GFP || Minipreped || --- || CBD test construct (T7)


|- style="background:#CCFFFF" align="center"
|- style="background:#CCFFFF" align="center"
||  || [http://parts.mit.edu/registry/index.php/Part:BBa_S03550 BBa_S03550] || RBS-AraC-noStop || Minipreped || ptet, pBad || CBD final construct (pBad)
||  || 12 ||[http://parts.mit.edu/registry/index.php/Part:BBa_S03550 BBa_S03550] || RBS-AraC-noStop || Minipreped || ptet, pBad || CBD final construct (pBad)


|- style="background:#CCFFFF" align="center"
|- style="background:#CCFFFF" align="center"
||  || [http://parts.mit.edu/registry/index.php/Part:BBa_B0015 BBa_B0015] || Double terminator || Minipreped || AraC || CBD test construct (pBad)
||  || 13 || [http://parts.mit.edu/registry/index.php/Part:BBa_B0015 BBa_B0015] || Double terminator || Minipreped || AraC || CBD test construct (pBad)


|- style="background:#CCFFFF" align="center"
|- style="background:#CCFFFF" align="center"
||  || [http://parts.mit.edu/registry/index.php/Part:BBa_R0051 BBa_R0051] || &lambda;cI regulated promoter || Minipreped || acGFP, DsRed || CBD final construct
||  || 14 || [http://parts.mit.edu/registry/index.php/Part:BBa_R0051 BBa_R0051] || &lambda;cI regulated promoter || Minipreped || acGFP, DsRed || CBD final construct


|- style="background:#CCFFFF" align="center"
|- style="background:#CCFFFF" align="center"
||  || [http://parts.mit.edu/registry/index.php/Part:BBa_J07037 BBa_J07037] || cI and GFP || Minipreped || --- || ''Not being used anymore''
||  || 16 || [http://parts.mit.edu/registry/index.php/Part:BBa_E0240 BBa_E0240] || GFP || Cloning || cI || CBD test construct (cI)


|- style="background:#CCFFFF" align="center"
|- style="background:#CCFFFF" align="center"
||  || [http://parts.mit.edu/registry/index.php/Part:BBa_E0240 BBa_E0240] || GFP || Not cloned || cI || CBD test construct (cI)
||  || 17 || [http://parts.mit.edu/registry/index.php/Part:BBa_I13507 BBa_I13507] || RFP || Cloning || CBD promoters || CBD final reporter


|- style="background:#CCFFFF" align="center"
|- style="background:#CCFFFF" align="center"
||  || [http://parts.mit.edu/registry/index.php/Part:BBa_I13507 BBa_I13507] || RFP || Not cloned || CBD promoters || CBD final reporter
||  || 18 || [http://parts.mit.edu/registry/index.php/Part:BBa_T9002 BBa_T9002] || ptet-luxR-plux-GFP || Cloning || --- || Basic construct for chassis characterization


|- style="background:#CCFFFF" align="center"
|- style="background:#CCFFFF" align="center"
||  || [http://parts.mit.edu/registry/index.php/Part:BBa_T9002 BBa_T9002] || ptet-luxR-plux-GFP || Not cloned || --- || Basic construct for chassis characterization
||  || 19 || [http://parts.mit.edu/registry/index.php/Part:BBa_B0034 BBa_B0034] || strong RBS || Cloning || --- || Backup part


|- style="background:#CCFFFF" align="center"
|- style="background:#CCFFFF" align="center"
||  || [http://parts.mit.edu/registry/index.php/Part:BBa_B0034 BBa_B0034] || strong RBS || Not cloned || --- || Backup part
||  || || BBa_I719??? || T7 promoter || Pending || acGFP, DsRed || CBD final construct


|- style="background:#CCFFFF" align="center"
|- style="background:#CCFFFF" align="center"
||  || BBa_I719??? || T7 promoter || Pending || acGFP, DsRed || CBD final construct
||  ||  || BBa_I719??? || acGFP || Ordered || ptet, pBad, plux || Reporter system for test constructs, Hrp


|- style="background:#CCFFFF" align="center"
|- style="background:#CCFFFF" align="center"
||  || BBa_I719??? || acGFP || Ordered || ptet, pBad, plux || Reporter system for test constructs, Hrp
||  ||  || BBa_I719??? || DsRed || Ordered || ptet, pBad, plux || Reporter system for ID, Hrp


|- style="background:#CCFFFF" align="center"
|- style="background:#CCFFFF" align="center"
||  || BBa_I719??? || DsRed || Ordered || ptet, pBad, plux || Reporter system for ID, Hrp
||  ||  || BBa_I719??? || HrpR-HrpS-pHrpL || Ordered || Promoter, acGFP || Hrp core system


|- style="background:#CCFFFF" align="center"
|- style="background:#CCFFFF" align="center"
||  || BBa_I719??? || HrpR-HrpS-pHrpL || Ordered || Promoter, acGFP || Hrp core system
||  ||  || BBa_I719??? || HrpV|| Ordered || Promoter, DsRed || Hrp inhibitor system


|- style="background:#CCFFFF" align="center"
|- style="background:#CCFFFF" align="center"
||  || BBa_I719??? || HrpV|| Ordered || Promoter, DsRed || Hrp inhibitor system
||  ||  || BBa_I719??? || lacZ || Stopped || --- || ''Not being used anymore''


|- style="background:#CCFFFF" align="center"
|- style="background:#CCFFFF" align="center"
||  || BBa_I719??? || lacZ || Stopped || --- || ''Not being used anymore''
||  || || || || ||  ||  
 
|}
|}


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|- style="background:#CCFFCC" align="center"
|- style="background:#CCFFCC" align="center"
|width=10px|I
|width=10px|I
|width=20px|ID
|width=10%|Part
|width=10%|Part
|width=20%|Description
|width=20%|Description
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|- style="background:#CCFFCC" align="center"
|- style="background:#CCFFCC" align="center"
||  || BBa_I719??? || RBS-araC & stop codon || Pending || [http://parts.mit.edu/registry/index.php/Part:BBa_S03550 BBa_S03550] + [http://parts.mit.edu/registry/index.php/Part:BBa_B0015 BBa_B0015] || CBD test construct 3 and final constructs 5, 8 and 10
||  ||  || BBa_I719??? || RBS-araC & stop codon || Pending || [http://parts.mit.edu/registry/index.php/Part:BBa_S03550 BBa_S03550] + [http://parts.mit.edu/registry/index.php/Part:BBa_B0015 BBa_B0015] || CBD test construct 3 and final constructs 5, 8 and 10


|- style="background:#CCFFCC" align="center"
|- style="background:#CCFFCC" align="center"
||  || BBa_I719??? || Ptet & araC || Pending || [http://parts.mit.edu/registry/index.php/Part:BBa_R0040 BBa_R0040] + araC with stop || CBD test construct 3 and final construct 8
||  ||  || BBa_I719??? || Ptet & araC || Pending || [http://parts.mit.edu/registry/index.php/Part:BBa_R0040 BBa_R0040] + araC with stop || CBD test construct 3 and final construct 8


|- style="background:#CCFFCC" align="center"
|- style="background:#CCFFCC" align="center"
||  || BBa_I719??? || Ptet-araC & pBad-gfp || Pending || Ptet-araC + [http://parts.mit.edu/registry/index.php/Part:BBa_J5528 BBa_J5528] || CBD test construct 3
||  ||  || BBa_I719??? || Ptet-araC & pBad-gfp || Pending || Ptet-araC + [http://parts.mit.edu/registry/index.php/Part:BBa_J5528 BBa_J5528] || CBD test construct 3


|- style="background:#CCFFCC" align="center"
|- style="background:#CCFFCC" align="center"
||  || BBa_I719??? || PcI & gfp || Pending || [http://parts.mit.edu/registry/index.php/Part:BBa_R0051 BBa_R0051] +  [http://parts.mit.edu/registry/index.php/Part:BBa_E0240 BBa_E0240] || CBD test construct 4
||  ||  || BBa_I719??? || PcI & gfp || Pending || [http://parts.mit.edu/registry/index.php/Part:BBa_R0051 BBa_R0051] +  [http://parts.mit.edu/registry/index.php/Part:BBa_E0240 BBa_E0240] || CBD test construct 4


|- style="background:#CCFFCC" align="center"
|- style="background:#CCFFCC" align="center"
||  || BBa_I719??? || PcI & araC || Pending || [http://parts.mit.edu/registry/index.php/Part:BBa_R0051 BBa_R0051] + araC with stop || CBD test construct 5 and final construct 10
||  ||  || BBa_I719??? || PcI & araC || Pending || [http://parts.mit.edu/registry/index.php/Part:BBa_R0051 BBa_R0051] + araC with stop || CBD test construct 5 and final construct 10


|- style="background:#CCFFCC" align="center"
|- style="background:#CCFFCC" align="center"
||  || BBa_I719??? || PcI-araC & pBad-gfp || Pending || PcI-araC + [http://parts.mit.edu/registry/index.php/Part:BBa_J5528 BBa_J5528] || CBD test construct 5
||  ||  || BBa_I719??? || PcI-araC & pBad-gfp || Pending || PcI-araC + [http://parts.mit.edu/registry/index.php/Part:BBa_J5528 BBa_J5528] || CBD test construct 5


|- style="background:#CCFFCC" align="center"
|- style="background:#CCFFCC" align="center"
||  || BBa_I719??? || pT7 & DsRed || Pending || pT7 + DsRed || CBD final construct 6
||  ||  || BBa_I719??? || pT7 & DsRed || Pending || pT7 + DsRed || CBD final construct 6


|- style="background:#CCFFCC" align="center"
|- style="background:#CCFFCC" align="center"
||  || BBa_I719??? || ptet & DsRed || Pending || [http://parts.mit.edu/registry/index.php/Part:BBa_R0040 BBa_R0040]+ DsRed || CBD final construct 7
||  ||  || BBa_I719??? || ptet & DsRed || Pending || [http://parts.mit.edu/registry/index.php/Part:BBa_R0040 BBa_R0040]+ DsRed || CBD final construct 7


|- style="background:#CCFFCC" align="center"
|- style="background:#CCFFCC" align="center"
||  || BBa_I719??? || pBad & DsRed || Pending || [http://parts.mit.edu/registry/index.php/Part:BBa_I0500 BBa_I0500] + DsRed || CBD final construct 8 and 10
||  ||  || BBa_I719??? || pBad & DsRed || Pending || [http://parts.mit.edu/registry/index.php/Part:BBa_I0500 BBa_I0500] + DsRed || CBD final construct 8 and 10


|- style="background:#CCFFCC" align="center"
|- style="background:#CCFFCC" align="center"
||  || BBa_I719??? || Ptet-araC & pBad-DsRed || Pending || Ptet-araC + pBad-DsRed || CBD final construct 8
||  ||  || BBa_I719??? || Ptet-araC & pBad-DsRed || Pending || Ptet-araC + pBad-DsRed || CBD final construct 8


|- style="background:#CCFFCC" align="center"
|- style="background:#CCFFCC" align="center"
||  || BBa_I719??? || pcI-DsRed || Pending || [http://parts.mit.edu/registry/index.php/Part:BBa_R0051 BBa_R0051] + DsRed || CBD final construct 9
||  ||  || BBa_I719??? || pcI-DsRed || Pending || [http://parts.mit.edu/registry/index.php/Part:BBa_R0051 BBa_R0051] + DsRed || CBD final construct 9


|- style="background:#CCFFCC" align="center"
|- style="background:#CCFFCC" align="center"
||  || BBa_I719??? || PcI-araC & pBad-DsRed || Pending || PcI-araC + pBad-DsRed || CBD final construct 10
||  ||  || BBa_I719??? || PcI-araC & pBad-DsRed || Pending || PcI-araC + pBad-DsRed || CBD final construct 10


|- style="background:#CCFFCC" align="center"
|- style="background:#CCFFCC" align="center"
||  || BBa_I719??? || ptet & luxI || Pending || [http://parts.mit.edu/registry/index.php/Part:BBa_R0040 R0040] + [http://parts.mit.edu/registry/index.php/Part:BBa_F1610 F1610] || ID AHL sender final construct 1
||  ||  || BBa_I719??? || ptet & luxI || Pending || [http://parts.mit.edu/registry/index.php/Part:BBa_R0040 R0040] + [http://parts.mit.edu/registry/index.php/Part:BBa_F1610 F1610] || ID AHL sender final construct 1


|- style="background:#CCFFCC" align="center"
|- style="background:#CCFFCC" align="center"
||  || BBa_I719??? || hrpR & hrpS|| Pending || hrpR + hrpS || ID AHL detector test construct 3 and final construct 5 (already got this??)
||  ||  || BBa_I719??? || hrpR & hrpS|| Pending || hrpR + hrpS || ID AHL detector test construct 3 and final construct 5 (already got this??)


|- style="background:#CCFFCC" align="center"
|- style="background:#CCFFCC" align="center"
||  || BBa_I719??? || hrpR-hrpS & phrpL|| Pending ||hrpR-hrpS + phrpL || ID AHL detector test construct 3 and final construct 5
||  ||  || BBa_I719??? || hrpR-hrpS & phrpL|| Pending ||hrpR-hrpS + phrpL || ID AHL detector test construct 3 and final construct 5


|- style="background:#CCFFCC" align="center"
|- style="background:#CCFFCC" align="center"
||  || BBa_I719??? || Ptet-luxR-Plux & hrpR-hrpS-phrpL|| Pending || [http://parts.mit.edu/registry/index.php/Part:BBa_F2620 BBa_F2620] + hrpR-hrpS-phrpL || ID AHL detector test construct 3 and final construct 5
||  ||  || BBa_I719??? || Ptet-luxR-Plux & hrpR-hrpS-phrpL|| Pending || [http://parts.mit.edu/registry/index.php/Part:BBa_F2620 BBa_F2620] + hrpR-hrpS-phrpL || ID AHL detector test construct 3 and final construct 5


|- style="background:#CCFFCC" align="center"
|- style="background:#CCFFCC" align="center"
||  || BBa_I719??? || Ptet-luxR-Plux-hrpR-hrpS-phrpL & GFP|| Pending ||Ptet-luxR-Plux-hrpR-hrpS-phrpL + [http://parts.mit.edu/registry/index.php/Part:BBa_E0240 BBa_E0240] || ID AHL detector test construct 3
||  ||  || BBa_I719??? || Ptet-luxR-Plux-hrpR-hrpS-phrpL & GFP|| Pending ||Ptet-luxR-Plux-hrpR-hrpS-phrpL + [http://parts.mit.edu/registry/index.php/Part:BBa_E0240 BBa_E0240] || ID AHL detector test construct 3


|- style="background:#CCFFCC" align="center"
|- style="background:#CCFFCC" align="center"
||  || BBa_I719??? || RBS & DsRed || Pending || [http://parts.mit.edu/registry/index.php/Part:BBa_B0034 BBa_B0034] + DsRed || ID AHL detector final construct 4 and 5
||  ||  || BBa_I719??? || RBS & DsRed || Pending || [http://parts.mit.edu/registry/index.php/Part:BBa_B0034 BBa_B0034] + DsRed || ID AHL detector final construct 4 and 5


|- style="background:#CCFFCC" align="center"
|- style="background:#CCFFCC" align="center"
||  || BBa_I719??? || RBS-DsRed & stop || Pending || RBS-DsRed + [http://parts.mit.edu/registry/index.php/Part:BBa_B0015 BBa_B0015] || ID AHL detector final construct 4 and 5
||  ||  || BBa_I719??? || RBS-DsRed & stop || Pending || RBS-DsRed + [http://parts.mit.edu/registry/index.php/Part:BBa_B0015 BBa_B0015] || ID AHL detector final construct 4 and 5


|- style="background:#CCFFCC" align="center"
|- style="background:#CCFFCC" align="center"
||  || BBa_I719??? || ptet-luxR-plux-DsRed || Pending || [http://parts.mit.edu/registry/index.php/Part:BBa_F2620 BBa_F2620] + DsRed (with RBS and stop)|| ID AHL detector final construct 4
||  ||  || BBa_I719??? || ptet-luxR-plux-DsRed || Pending || [http://parts.mit.edu/registry/index.php/Part:BBa_F2620 BBa_F2620] + DsRed (with RBS and stop)|| ID AHL detector final construct 4


|- style="background:#CCFFCC" align="center"
|- style="background:#CCFFCC" align="center"
||  || BBa_I719??? || Ptet-luxR-Plux-hrpR-hrpS-phrpL & DsRed|| Pending ||Ptet-luxR-Plux-hrpR-hrpS-phrpL + DsRed (with RBS and stop) || ID AHL detector final construct 5
||  ||  || BBa_I719??? || Ptet-luxR-Plux-hrpR-hrpS-phrpL & DsRed|| Pending ||Ptet-luxR-Plux-hrpR-hrpS-phrpL + DsRed (with RBS and stop) || ID AHL detector final construct 5


|- style="background:#CCFFCC" align="center"
|- style="background:#CCFFCC" align="center"
||  ||  ||  ||  ||  ||
||  ||  ||  ||  ||  ||  ||
|}
|}


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|width=10px| G
|width=10px| G
|width=10px| S
|width=10px| S
|width=20px|ID
|width=10%| Name
|width=10%| Name
|witdh="--"| Description
|witdh="--"| Description
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|width=12%| Location
|width=12%| Location
|- style="background:#FFCCFF" align="center"
|- style="background:#FFCCFF" align="center"
|| A || W || G ||  || [http://parts.mit.edu/registry/index.php?title=Part:BBa_E0040 BBa_E0040] || GFP || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 720bp || 2799bp || Well 5H, Plate 1
|| A || W || G ||  || 01 || [http://parts.mit.edu/registry/index.php?title=Part:BBa_E0040 BBa_E0040] || GFP || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 720bp || 2799bp || Well 5H, Plate 1


|- style="background:#FFCCFF" align="center"
|- style="background:#FFCCFF" align="center"
|| A || W || G ||  || [http://parts.mit.edu/registry/index.php/Part:BBa_I13522 BBa_I13522] || GFP under tetracyclin promoter || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 937bp || 3016bp || Well 15H, Plate 2
|| A || W || G ||  || 02 || [http://parts.mit.edu/registry/index.php/Part:BBa_I13522 BBa_I13522] || GFP under tetracyclin promoter || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 937bp || 3016bp || Well 15H, Plate 2


|- style="background:#FFCCFF" align="center"
|- style="background:#FFCCFF" align="center"
|| A ||  ||  ||  || [http://parts.mit.edu/registry/index.php/Part:BBa_J5528 BBa_J5528] || GFP under arabinose promoter || [http://parts.mit.edu/registry/index.php/Part:pSB2K3 pSB2K3] || KanR || Low || 2093bp || 6518bp || Well 6H, Plate 3
|| A ||  ||  ||  || 03 || [http://parts.mit.edu/registry/index.php/Part:BBa_J5528 BBa_J5528] || GFP under arabinose promoter || [http://parts.mit.edu/registry/index.php/Part:pSB2K3 pSB2K3] || KanR || Low || 2093bp || 6518bp || Well 6H, Plate 3


|- style="background:#FFCCFF" align="center"
|- style="background:#FFCCFF" align="center"
|| A || W || G ||  || [http://parts.mit.edu/registry/index.php/Part:BBa_J24677 BBa_J24677] || GFP under lux promoter, with luxR || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 1787bp || 3866bp || Well 18K, Plate 3
|| A || W || G ||  || 04 || [http://parts.mit.edu/registry/index.php/Part:BBa_J24677 BBa_J24677] || GFP under lux promoter, with luxR || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 1787bp || 3866bp || Well 18K, Plate 3
<!-- Number 05 -->
<!-- Number 05 -->
|- style="background:#FFCCFF" align="center"
|- style="background:#FFCCFF" align="center"
|| A || W || <font color=#990033>X</font> ||  || [http://parts.mit.edu/registry/index.php/Part:BBa_R0040 BBa_R0040] || tetracyclin promoter || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High ||  54bp || 2133bp || Well 7O, Plate 1
|| A || W || <font color=#990033>X</font> ||  || 05 || [http://parts.mit.edu/registry/index.php/Part:BBa_R0040 BBa_R0040] || tetracyclin promoter || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High ||  54bp || 2133bp || Well 7O, Plate 1


|- style="background:#FFCCFF" align="center"
|- style="background:#FFCCFF" align="center"
|| A || W || G ||  || [http://parts.mit.edu/registry/index.php/Part:BBa_I0500 BBa_I0500] || arabinose promoter || [http://parts.mit.edu/registry/index.php/Part:pSB2K3 pSB2K3] || KanR || Low || 1210bp || 5635bp || Well 9I, Plate 2
|| A || W || G ||  || 06 || [http://parts.mit.edu/registry/index.php/Part:BBa_I0500 BBa_I0500] || arabinose promoter || [http://parts.mit.edu/registry/index.php/Part:pSB2K3 pSB2K3] || KanR || Low || 1210bp || 5635bp || Well 9I, Plate 2


|- style="background:#FFCCFF" align="center"
|- style="background:#FFCCFF" align="center"
|| A || W || G ||  || [http://parts.mit.edu/registry/index.php/Part:BBa_F2620 BBa_F2620] || lux promoter, with luxR || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 1061bp || 3140bp || Well 6B, Plate 1
|| A || W || G ||  || 07 || [http://parts.mit.edu/registry/index.php/Part:BBa_F2620 BBa_F2620] || lux promoter, with luxR || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 1061bp || 3140bp || Well 6B, Plate 1


|- style="background:#FFCCFF" align="center"
|- style="background:#FFCCFF" align="center"
|| A || W || G ||  || [http://parts.mit.edu/registry/index.php/Part:BBa_F1610 BBa_F1610] || luxI coding region || [http://parts.mit.edu/registry/index.php/Part:pSB1AK3 pSB1AK3] || A+KR ||  High || 798bp || 3987bp || Well 1B, Plate 2
|| A || W || G ||  || 08 || [http://parts.mit.edu/registry/index.php/Part:BBa_F1610 BBa_F1610] || luxI coding region || [http://parts.mit.edu/registry/index.php/Part:pSB1AK3 pSB1AK3] || A+KR ||  High || 798bp || 3987bp || Well 1B, Plate 2


|- style="background:#FFCCFF" align="center"
|- style="background:#FFCCFF" align="center"
|| A || W || G ||  || [http://parts.mit.edu/registry/index.php/Part:BBa_R0010 BBa_R0010] || lac promoter || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 200bp || 2279bp || Well 7K, Plate 1
|| A || W || G ||  || 09 || [http://parts.mit.edu/registry/index.php/Part:BBa_R0010 BBa_R0010] || lac promoter || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 200bp || 2279bp || Well 7K, Plate 1
<!-- Number 10 -->
<!-- Number 10 -->
|- style="background:#FFCCFF" align="center"
|- style="background:#FFCCFF" align="center"
|| A ||  || G ||  || [http://parts.mit.edu/registry/index.php/Part:BBa_E1010 BBa_E1010] || RFP || [http://parts.mit.edu/registry/index.php/Part:pSB2K3 pSB2K3] || KanR ||  Low || 681bp || 5106bp || Well 15M, Plate 2
|| A ||  || G ||  || 10 || [http://parts.mit.edu/registry/index.php/Part:BBa_E1010 BBa_E1010] || RFP || [http://parts.mit.edu/registry/index.php/Part:pSB2K3 pSB2K3] || KanR ||  Low || 681bp || 5106bp || Well 15M, Plate 2
 
|- style="background:#FFCCFF" align="center"
|| A || W || G ||  || [http://parts.mit.edu/registry/index.php/Part:BBa_E7104 BBa_E7104] || T7 GFP|| [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 826bp || 2905bp || Well 13F, Plate 2  


|- style="background:#FFCCFF" align="center"
|- style="background:#FFCCFF" align="center"
|| A || || <font color=#339900>G</font> ||  || [http://parts.mit.edu/registry/index.php/Part:BBa_S03550 BBa_S03550] || AraC with RBS but no stop || [http://parts.mit.edu/registry/index.php/Part:pSB1AC3 pSB1AC3] || A+CR || High || 958bp || 4013bp || Well 20E, Plate 3
|| A || W || G ||  || 11 || [http://parts.mit.edu/registry/index.php/Part:BBa_E7104 BBa_E7104] || T7 GFP|| [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 826bp || 2905bp || Well 13F, Plate 2


|- style="background:#FFCCFF" align="center"
|- style="background:#FFCCFF" align="center"
|| A || W || G ||  || [http://parts.mit.edu/registry/index.php/Part:BBa_B0015 BBa_B0015] || Double terminator || [http://parts.mit.edu/registry/index.php/Part:pSB1AK3 pSB1AK3] || A+KR || High || 129bp || 3316bp || Well 1B, Plate 2
|| A || || <font color=#339900>G</font> ||  || 12 || [http://parts.mit.edu/registry/index.php/Part:BBa_S03550 BBa_S03550] || AraC with RBS but no stop || [http://parts.mit.edu/registry/index.php/Part:pSB1AC3 pSB1AC3] || A+CR || High || 958bp || 4013bp || Well 20E, Plate 3


|- style="background:#FFCCFF" align="center"
|- style="background:#FFCCFF" align="center"
|| A || W || <font color=#990033>X</font> ||  || [http://parts.mit.edu/registry/index.php/Part:BBa_R0051 BBa_R0051] || &lambda;cI promoter || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 49bp || 2128bp || Well 9C, Plate 1
|| A || W || G ||  || 13 || [http://parts.mit.edu/registry/index.php/Part:BBa_B0015 BBa_B0015] || Double terminator || [http://parts.mit.edu/registry/index.php/Part:pSB1AK3 pSB1AK3] || A+KR || High || 129bp || 3316bp || Well 1B, Plate 2


|- style="background:#FFCCFF" align="center"
|- style="background:#FFCCFF" align="center"
|| A || || <font color=#339900>G</font> ||  || [http://parts.mit.edu/registry/index.php/Part:BBa_J07037 BBa_J07037] || GFP under cI promoter || [http://parts.mit.edu/registry/index.php/Part:pSB2K3 pSB2K3] || KanR || Low || 912bp || 5337bp || Well 12D, Plate 2
|| A || W || <font color=#990033>X</font> ||  || 14 || [http://parts.mit.edu/registry/index.php/Part:BBa_R0051 BBa_R0051] || &lambda;cI promoter || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 49bp || 2128bp || Well 9C, Plate 1


|- style="background:#FFCCFF" align="center"
|- style="background:#FFCCFF" align="center"
|| A ||  ||  ||  || [http://parts.mit.edu/registry/index.php/Part:BBa_E0240 BBa_E0240] || GFP || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 876bp || 2955bp || Well 16A, Plate 1
|| A ||  ||  ||  || 16 || [http://parts.mit.edu/registry/index.php/Part:BBa_E0240 BBa_E0240] || GFP || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 876bp || 2955bp || Well 16A, Plate 1


|- style="background:#FFCCFF" align="center"
|- style="background:#FFCCFF" align="center"
|| A ||  ||  ||  || [http://parts.mit.edu/registry/index.php/Part:BBa_I13507 BBa_I13507] || RFP || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 861bp || 2940bp || Well 16N, Plate 1
|| A ||  ||  ||  || 17 || [http://parts.mit.edu/registry/index.php/Part:BBa_I13507 BBa_I13507] || RFP || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 861bp || 2940bp || Well 16N, Plate 1


|- style="background:#FFCCFF" align="center"
|- style="background:#FFCCFF" align="center"
|| A || W ||  ||  || [http://parts.mit.edu/registry/index.php/Part:BBa_T9002 BBa_T9002] || GFP under lux promoter, with luxR || [http://parts.mit.edu/registry/index.php/Part:pSB1A3 pSB1A3] || AmpR || High ||  1945bp || 4102bp || Well 19B, Plate 2
|| A || W ||  ||  || 18 || [http://parts.mit.edu/registry/index.php/Part:BBa_T9002 BBa_T9002] || GFP under lux promoter, with luxR || [http://parts.mit.edu/registry/index.php/Part:pSB1A3 pSB1A3] || AmpR || High ||  1945bp || 4102bp || Well 19B, Plate 2


|- style="background:#FFCCFF" align="center"
|- style="background:#FFCCFF" align="center"
|| A || W ||  ||  || [http://parts.mit.edu/registry/index.php/Part:BBa_B0034 BBa_B0034] || RBS || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 12bp || 2091bp || Well 3O, Plate 1
|| A || W ||  ||  || 19 || [http://parts.mit.edu/registry/index.php/Part:BBa_B0034 BBa_B0034] || RBS || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 12bp || 2091bp || Well 3O, Plate 1


|- style="background:#FFCCFF" align="center"
|- style="background:#FFCCFF" align="center"
||  ||  ||  ||  ||  ||  ||  ||  ||  ||  ||  ||  
||  ||  ||  ||  ||  ||  ||  ||  ||  ||  ||  ||  ||  
|}
|}
<font size="-2">Legend: A (Available), W (Working), G (Checked on gel) [<font color=#339900>G</font> - incorrect size; <font color=#990033>X</font> - does not appear on gel], S (Sequenced)</font>
<font size="-2">Legend: A (Available), W (Working), G (Checked on gel) [<font color=#339900>G</font> - incorrect size; <font color=#990033>X</font> - does not appear on gel], S (Sequenced)</font>
Line 251: Line 250:
|width=10px| G
|width=10px| G
|width=10px| S
|width=10px| S
|width=20px| ID
|width=10%| Name
|width=10%| Name
|witdh="--"| Description
|witdh="--"| Description
Line 260: Line 260:
|width=12%| Stock
|width=12%| Stock
|- style="background:#FFFFBB" align="center"
|- style="background:#FFFFBB" align="center"
||  ||  ||  ||  || BBa_I791??? || luxI coding region under tetracyclin promoter || pSB1A2 || AmpR || High || 860bp || 2939bp ||
||  ||  ||  ||  ||  || BBa_I791??? || luxI coding region under tetracyclin promoter || pSB1A2 || AmpR || High || 860bp || 2939bp ||


|- style="background:#FFFFBB" align="center"
|- style="background:#FFFFBB" align="center"
||  ||  ||  ||  || BBa_I791??? || DsRed under tetracyclin promoter || pSB1A2 || AmpR || High ||  ||  ||
||  ||  ||  ||  ||  || BBa_I791??? || DsRed under tetracyclin promoter || pSB1A2 || AmpR || High ||  ||  ||


|- style="background:#FFFFBB" align="center"
|- style="background:#FFFFBB" align="center"
||  ||  ||  ||  || BBa_I791??? || DsRed under arabinose promoter || pSB2K3 || KanR || Low ||  ||  ||
||  ||  ||  ||  ||  || BBa_I791??? || DsRed under arabinose promoter || pSB2K3 || KanR || Low ||  ||  ||


|- style="background:#FFFFBB" align="center"
|- style="background:#FFFFBB" align="center"
||  ||  ||  ||  ||  ||  ||  ||  ||  ||  ||  ||
||  ||  ||  ||  ||  ||  ||  ||  ||  ||  ||  ||  ||
|}
|}


Line 277: Line 277:
|width=10px| A
|width=10px| A
|width=10px| W
|width=10px| W
|width=20px| ID
|width=10%| Name
|width=10%| Name
|witdh=30%| Description
|witdh=30%| Description
Line 284: Line 285:
|width="--"%| Notes
|width="--"%| Notes
|- style="background:#DDDDDD" align="center"
|- style="background:#DDDDDD" align="center"
|| A ||  || BBa_J07037 || cI-GFP || pSB2K3 || 912bp || Miniprepped || No RBS present on this part
|| A ||  || 15 || BBa_J07037 || cI-GFP || pSB2K3 || 912bp || Miniprepped || No RBS present on this part


|- style="background:#DDDDDD" align="center"
|- style="background:#DDDDDD" align="center"

Revision as of 03:20, 14 August 2007

Constructs Overview

Cloning Table

I ID Part Description Status Ligation Target Use in Constructs
01 BBa_E0040 GFP Minipreped --- Not being used anymore
02 BBa_I13522 ptet-GFP Minipreped --- Basic construct for chassis characterization
03 BBa_J5528 pBad-GFP Cloning --- Test construct for CBD (pBad)
I 04 BBa_J24677 ptet-luxR-plux-GFP Minipreped --- Not being used anymore
05 BBa_R0040 ptet Minipreped acGFP, DsRed, luxI CBD final (pBad), ID AHL sender, Hrp test construct
I 06 BBa_I0500 pBad Minipreped acGFP, DsRed CBD final construct, Hrp test construct
07 BBa_F2620 ptet-luxR-plux Minipreped lacZ, Hrp amplifier ID final construct
08 BBa_F1610 luxI Minipreped ptet ID AHL sender final construct
09 BBa_R0010 lacI-pLac Minipreped --- Not being used anymore
10 BBa_E1010 RFP Minipreped --- Not being used anymore
11 BBa_E7104 T7 GFP Minipreped --- CBD test construct (T7)
12 BBa_S03550 RBS-AraC-noStop Minipreped ptet, pBad CBD final construct (pBad)
13 BBa_B0015 Double terminator Minipreped AraC CBD test construct (pBad)
14 BBa_R0051 λcI regulated promoter Minipreped acGFP, DsRed CBD final construct
16 BBa_E0240 GFP Cloning cI CBD test construct (cI)
17 BBa_I13507 RFP Cloning CBD promoters CBD final reporter
18 BBa_T9002 ptet-luxR-plux-GFP Cloning --- Basic construct for chassis characterization
19 BBa_B0034 strong RBS Cloning --- Backup part
BBa_I719??? T7 promoter Pending acGFP, DsRed CBD final construct
BBa_I719??? acGFP Ordered ptet, pBad, plux Reporter system for test constructs, Hrp
BBa_I719??? DsRed Ordered ptet, pBad, plux Reporter system for ID, Hrp
BBa_I719??? HrpR-HrpS-pHrpL Ordered Promoter, acGFP Hrp core system
BBa_I719??? HrpV Ordered Promoter, DsRed Hrp inhibitor system
BBa_I719??? lacZ Stopped --- Not being used anymore

Ligation Table

I ID Part Description Status Parents Application


BBa_I719??? RBS-araC & stop codon Pending BBa_S03550 + BBa_B0015 CBD test construct 3 and final constructs 5, 8 and 10
BBa_I719??? Ptet & araC Pending BBa_R0040 + araC with stop CBD test construct 3 and final construct 8
BBa_I719??? Ptet-araC & pBad-gfp Pending Ptet-araC + BBa_J5528 CBD test construct 3
BBa_I719??? PcI & gfp Pending BBa_R0051 + BBa_E0240 CBD test construct 4
BBa_I719??? PcI & araC Pending BBa_R0051 + araC with stop CBD test construct 5 and final construct 10
BBa_I719??? PcI-araC & pBad-gfp Pending PcI-araC + BBa_J5528 CBD test construct 5
BBa_I719??? pT7 & DsRed Pending pT7 + DsRed CBD final construct 6
BBa_I719??? ptet & DsRed Pending BBa_R0040+ DsRed CBD final construct 7
BBa_I719??? pBad & DsRed Pending BBa_I0500 + DsRed CBD final construct 8 and 10
BBa_I719??? Ptet-araC & pBad-DsRed Pending Ptet-araC + pBad-DsRed CBD final construct 8
BBa_I719??? pcI-DsRed Pending BBa_R0051 + DsRed CBD final construct 9
BBa_I719??? PcI-araC & pBad-DsRed Pending PcI-araC + pBad-DsRed CBD final construct 10
BBa_I719??? ptet & luxI Pending R0040 + F1610 ID AHL sender final construct 1
BBa_I719??? hrpR & hrpS Pending hrpR + hrpS ID AHL detector test construct 3 and final construct 5 (already got this??)
BBa_I719??? hrpR-hrpS & phrpL Pending hrpR-hrpS + phrpL ID AHL detector test construct 3 and final construct 5
BBa_I719??? Ptet-luxR-Plux & hrpR-hrpS-phrpL Pending BBa_F2620 + hrpR-hrpS-phrpL ID AHL detector test construct 3 and final construct 5
BBa_I719??? Ptet-luxR-Plux-hrpR-hrpS-phrpL & GFP Pending Ptet-luxR-Plux-hrpR-hrpS-phrpL + BBa_E0240 ID AHL detector test construct 3
BBa_I719??? RBS & DsRed Pending BBa_B0034 + DsRed ID AHL detector final construct 4 and 5
BBa_I719??? RBS-DsRed & stop Pending RBS-DsRed + BBa_B0015 ID AHL detector final construct 4 and 5
BBa_I719??? ptet-luxR-plux-DsRed Pending BBa_F2620 + DsRed (with RBS and stop) ID AHL detector final construct 4
BBa_I719??? Ptet-luxR-Plux-hrpR-hrpS-phrpL & DsRed Pending Ptet-luxR-Plux-hrpR-hrpS-phrpL + DsRed (with RBS and stop) ID AHL detector final construct 5

Legend: I (Information)

Parts from the Registry

A W G S ID Name Description Plasmid Resist Copy Size(B) Size(T) Location
A W G 01 BBa_E0040 GFP pSB1A2 AmpR High 720bp 2799bp Well 5H, Plate 1
A W G 02 BBa_I13522 GFP under tetracyclin promoter pSB1A2 AmpR High 937bp 3016bp Well 15H, Plate 2
A 03 BBa_J5528 GFP under arabinose promoter pSB2K3 KanR Low 2093bp 6518bp Well 6H, Plate 3
A W G 04 BBa_J24677 GFP under lux promoter, with luxR pSB1A2 AmpR High 1787bp 3866bp Well 18K, Plate 3
A W X 05 BBa_R0040 tetracyclin promoter pSB1A2 AmpR High 54bp 2133bp Well 7O, Plate 1
A W G 06 BBa_I0500 arabinose promoter pSB2K3 KanR Low 1210bp 5635bp Well 9I, Plate 2
A W G 07 BBa_F2620 lux promoter, with luxR pSB1A2 AmpR High 1061bp 3140bp Well 6B, Plate 1
A W G 08 BBa_F1610 luxI coding region pSB1AK3 A+KR High 798bp 3987bp Well 1B, Plate 2
A W G 09 BBa_R0010 lac promoter pSB1A2 AmpR High 200bp 2279bp Well 7K, Plate 1
A G 10 BBa_E1010 RFP pSB2K3 KanR Low 681bp 5106bp Well 15M, Plate 2
A W G 11 BBa_E7104 T7 GFP pSB1A2 AmpR High 826bp 2905bp Well 13F, Plate 2
A G 12 BBa_S03550 AraC with RBS but no stop pSB1AC3 A+CR High 958bp 4013bp Well 20E, Plate 3
A W G 13 BBa_B0015 Double terminator pSB1AK3 A+KR High 129bp 3316bp Well 1B, Plate 2
A W X 14 BBa_R0051 λcI promoter pSB1A2 AmpR High 49bp 2128bp Well 9C, Plate 1
A 16 BBa_E0240 GFP pSB1A2 AmpR High 876bp 2955bp Well 16A, Plate 1
A 17 BBa_I13507 RFP pSB1A2 AmpR High 861bp 2940bp Well 16N, Plate 1
A W 18 BBa_T9002 GFP under lux promoter, with luxR pSB1A3 AmpR High 1945bp 4102bp Well 19B, Plate 2
A W 19 BBa_B0034 RBS pSB1A2 AmpR High 12bp 2091bp Well 3O, Plate 1

Legend: A (Available), W (Working), G (Checked on gel) [G - incorrect size; X - does not appear on gel], S (Sequenced)

Our Parts

A W G S ID Name Description Plasmid Resist Copy Size(B) Size(P) Stock
BBa_I791??? luxI coding region under tetracyclin promoter pSB1A2 AmpR High 860bp 2939bp
BBa_I791??? DsRed under tetracyclin promoter pSB1A2 AmpR High
BBa_I791??? DsRed under arabinose promoter pSB2K3 KanR Low

Part Graveyard

A W ID Name Description Plasmid Size Status Notes
A 15 BBa_J07037 cI-GFP pSB2K3 912bp Miniprepped No RBS present on this part

Constructs for Cell By Date

  • Construct CBD 1
    • Entire construct is currently available in Biobricks Registry: BBa_E7104 (available), in pSB1A2 (AmpR)
    • pT7 can be used with T7 RNAP in vitro and in veso, and it gives constitutive expression.
PT7 promoting GFP


  • Construct CBD 2
    • Entire construct is currently in Biobricks Registry: BBa_I13522 (available), in pSB1A2 (AmpR)
    • pTet can be used with E.coli RNAP in vitro and maybe in veso, and it gives constitutive expression.
Ptet promoting GFP


  • Construct CBD 3
    • pBAD promoter with GFP is currently available in Biobricks Registry: BBa_J5528 (available), in pSB2K3 (KanR)
    • Need Ara C regulator protein in vitro and in veso. Ara C available with the RBS:BBa_S03550
    • pBad can be used with E.coli RNAP in vitro, and it is inducible by arabinose.
    • To be used in veso, arabinose transport proteins have to be inserted in the phospholipid bilayer of the vesicles.
PBAD promoting GFP and Ptet promoting araC


  • Construct CBD 4
    • Entire construct is currently available in Biobricks Registry: BBa_J07037 (avaiable), in pSB2K3 (KanR)
    • pcI gives constitutive expression.
PcI promoting GFP


PBAD promoting GFP and PcI promoting araC


  • Construct CBD 6
    • Parts avaiable ....
pT7 promoting RFP


  • Construct CBD 7
    • Parts avaiable ....
Ptet promoting RFP


  • Construct CBD 8
    • Parts avaiable ....
PBAD promoting RFP and Ptet promoting araC


  • Construct CBD 9
    • Parts avaiable ....
pcI promoting RFP


  • Construct CBD 10
    • Parts avaiable ....
PBAD promoting RFP and PcI promoting araC


Constructs for Infector Detector

  • Construct ID 1
    • Entire part minus promoter is currently in Biobricks Registry: BBa_F1610 (available), in pSB1AK3 (AmpR, KanR)
    • pTet is currently in Biobricks Registry: BBa_R0040 (available), in pSB1A2 (AmpR)
    • pTet can be used with E.coli RNAP in vitro and maybe in veso, and it gives constitutive expression.
Ptet promoting LuxI


  • Construct ID 2
    • Entire part is currently in Biobricks Registry: BBa_J24677 (available), in pSB1A2 (AmpR)
    • Entire part minus GFP reporter system is currently in Biobricks Registry: BBa_F2620 (available), in pSB1A2 (AmpR)
    • GFP reporter system is currently in Biobricks Registry: BBa_I13504 (available)
    • pLux can be used with E.coli RNAP in vitro and maybe in veso, and it is repressed by LuxR but activated by AHL:LuxR complex.
Ptet promoting LuxR, Plux promoting GFP


  • Construct ID 3
    • Entire part minus Hrp system is currently in Biobricks Registry: BBa_F2620(available) in pSB1A2 (AmpR)
    • pHrpL can be used with E.coli RNAP in vitro and maybe in veso, and it is inducible by HrpR:HrpS:Sigma 54 complex.
Ptet promoting LuxR, Plux promoting HrpR and HrpS and PHrpL promoting GFP


  • Construct ID 4
    • Parts available ...
Ptet promoting LuxI


  • Construct ID 5
    • Parts available ...
Ptet promoting LuxR, Plux promoting HrpR and HrpS and PHrpL promoting lacZ


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