IGEM:IMPERIAL/2007/DNA Constructs: Difference between revisions
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|width=12%|Ligation Target | |width=12%|Ligation Target | ||
|width="--"%|Use in Constructs | |width="--"%|Use in Constructs | ||
<!-- PROMOTERS WITH GFP --> | |||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || 02 || [http://parts.mit.edu/registry/index.php/Part:BBa_I13522 BBa_I13522] || ptet-GFP || <font color=#CC0000>XL1</font>, <font color=#0000FF>'''Midi'''</font>, <font color=#00CC00>''Mini''</font>, <font color=#FF6633>BL21</font> || --- || Basic construct for chassis characterization | || || 02 || [http://parts.mit.edu/registry/index.php/Part:BBa_I13522 BBa_I13522] || ptet-GFP || <font color=#CC0000>XL1</font>, <font color=#0000FF>'''Midi'''</font>, <font color=#00CC00>''Mini''</font>, <font color=#FF6633>BL21</font> || --- || Basic construct for chassis characterization | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || 11 || [http://parts.mit.edu/registry/index.php/Part:BBa_E7104 BBa_E7104] || | || || 11 || [http://parts.mit.edu/registry/index.php/Part:BBa_E7104 BBa_E7104] || pT7 GFP || <font color=#CC0000>XL1</font>, <font color=#0000FF>'''Midi'''</font>, <font color=#00CC00>''Mini''</font>, <font color=#FF6633>BL21</font> || --- || CBD test construct (pT7) | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
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|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || 22 || [http://parts.mit.edu/registry/index.php/Part:BBa_S03335 BBa_S03335] || | || || 22 || [http://parts.mit.edu/registry/index.php/Part:BBa_S03335 BBa_S03335] || pcI-GFP || Not Cloned || --- || CBD test construct (pcI) | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || || || || || || | || || || || || || || | ||
<!-- PROMOTERS ONLY --> | |||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || 05 || [http://parts.mit.edu/registry/index.php/Part:BBa_R0040 BBa_R0040] || ptet || <font color=#CC0000>XL1</font>, <font color=#0000FF>'''Midi'''</font>, <font color=#00CC00>''Mini''</font> || DsRed || CBD final ( | || || 05 || [http://parts.mit.edu/registry/index.php/Part:BBa_R0040 BBa_R0040] || ptet || <font color=#CC0000>XL1</font>, <font color=#0000FF>'''Midi'''</font>, <font color=#00CC00>''Mini''</font> || DsRed || CBD final (ptet), ID AHL sender | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || || BBa_I719??? || | || || || BBa_I719??? || pT7 || Pending || DsRed || CBD final construct (pT7) | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
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|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || 14 || [http://parts.mit.edu/registry/index.php/Part:BBa_R0051 BBa_R0051] || | || || 14 || [http://parts.mit.edu/registry/index.php/Part:BBa_R0051 BBa_R0051] || pcI || <font color=#CC0000>XL1</font>, <font color=#0000FF>'''Midi'''</font>, <font color=#00CC00>''Mini''</font> || DsRed || CBD final construct (pcI) | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || || || || || || | || || || || || || || | ||
<!-- ID SPECIFIC BRICKS --> | |||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || 08 || [http://parts.mit.edu/registry/index.php/Part:BBa_F1610 BBa_F1610] || luxI || <font color=#CC0000>XL1</font>, <font color=#0000FF>'''Midi'''</font>, <font color=#00CC00>''Mini''</font> || ptet || ID AHL sender final construct | || || 08 || [http://parts.mit.edu/registry/index.php/Part:BBa_F1610 BBa_F1610] || luxI || <font color=#CC0000>XL1</font>, <font color=#0000FF>'''Midi'''</font>, <font color=#00CC00>''Mini''</font> || ptet || ID AHL sender final construct | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || 21 || [http://parts.mit.edu/registry/index.php/Part:BBa_J23039 BBa_J23039] || ptet- | || || 21 || [http://parts.mit.edu/registry/index.php/Part:BBa_J23039 BBa_J23039] || ptet-luxI || Not Cloned || --- || ID sender construct | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || || || || || || | || || || || || || || | ||
<!-- BRICKS FOR CONSTRUCTION [NOT USEFUL] --> | |||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || 01 || [http://parts.mit.edu/registry/index.php?title=Part:BBa_E0040 BBa_E0040] || GFP || <font color=#CC0000>XL1</font>, <font color=#00CC00>''Mini''</font> || --- || ''Not being used anymore'' | || || 01 || [http://parts.mit.edu/registry/index.php?title=Part:BBa_E0040 BBa_E0040] || GFP || <font color=#CC0000>XL1</font>, <font color=#00CC00>''Mini''</font> || --- || ''Not being used anymore'' | ||
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|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || || || || || || | || || || || || || || | ||
<!-- BRICKS BEING ORDERED --> | |||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || || BBa_I719??? || acGFP || Ordered || ptet, plux, | || || || BBa_I719??? || acGFP || Ordered || ptet, plux, pcI || Reporter system for test constructs, Hrp | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
|| || || BBa_I719??? || DsRed || Ordered || ptet, plux, | || || || BBa_I719??? || DsRed || Ordered || ptet, plux, pcI || Reporter system for ID, Hrp | ||
|- style="background:#CCFFFF" align="center" | |- style="background:#CCFFFF" align="center" | ||
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|- style="background:#CCFFCC" align="center" | |- style="background:#CCFFCC" align="center" | ||
|| || || [http://parts.mit.edu/registry/index.php/Part:BBa_I719022 BBa_I719022] || | || || || [http://parts.mit.edu/registry/index.php/Part:BBa_I719022 BBa_I719022] || pcI & GFP || ''Concerns'' || [http://parts.mit.edu/registry/index.php/Part:BBa_R0051 BBa_R0051] + [http://parts.mit.edu/registry/index.php/Part:BBa_E0240 BBa_E0240] || CBD test construct (pcI) | ||
|- style="background:#CCFFCC" align="center" | |- style="background:#CCFFCC" align="center" | ||
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|} | |} | ||
<font size="-2">Legend: I (Information)</font> | <font size="-2">Legend: I (Information), [Status: <font color=#CC0000>XL1</font> - cloned in XL1-Blue; <font color=#0000FF>'''Midi'''</font> - midiprepped; <font color=#00CC00>''Mini''</font> - miniprepped; <font color=#FF6633>BL21</font> - cloned in BL21(DE3)]</font> | ||
===Parts from the Registry=== | ===Parts from the Registry=== | ||
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|width=10%| Name | |width=10%| Name | ||
|witdh="--"| Description | |witdh="--"| Description | ||
|width= | |width=9%| Plasmid | ||
|width=55px| Resist | |width=55px| Resist | ||
|width=55px| Copy | |width=55px| Copy | ||
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|width=65px| Size(T) | |width=65px| Size(T) | ||
|width=12%| Location | |width=12%| Location | ||
|width=7%| Conc. | |||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
|| A || W || G || || 01 || [http://parts.mit.edu/registry/index.php?title=Part:BBa_E0040 BBa_E0040] || GFP || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 720bp || 2799bp || Well 5H, Plate 1 | || A || W || G || || 01 || [http://parts.mit.edu/registry/index.php?title=Part:BBa_E0040 BBa_E0040] || GFP || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 720bp || 2799bp || Well 5H, Plate 1 || -- | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
|| A || W || G || || 02 || [http://parts.mit.edu/registry/index.php/Part:BBa_I13522 BBa_I13522] || GFP under tetracyclin promoter || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 937bp || 3016bp || Well 15H, Plate 2 | || A || W || G || || 02 || [http://parts.mit.edu/registry/index.php/Part:BBa_I13522 BBa_I13522] || GFP under tetracyclin promoter || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 937bp || 3016bp || Well 15H, Plate 2 || -- | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
|| A || W || <font color=#990033>X</font> || || 05 || [http://parts.mit.edu/registry/index.php/Part:BBa_R0040 BBa_R0040] || tetracyclin promoter || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 54bp || 2133bp || Well 7O, Plate 1 | || A || W || <font color=#990033>X</font> || || 05 || [http://parts.mit.edu/registry/index.php/Part:BBa_R0040 BBa_R0040] || tetracyclin promoter || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 54bp || 2133bp || Well 7O, Plate 1 || -- | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
|| A || W || G || || 07 || [http://parts.mit.edu/registry/index.php/Part:BBa_F2620 BBa_F2620] || lux promoter, with luxR || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 1061bp || 3140bp || Well 6B, Plate 1 | || A || W || G || || 07 || [http://parts.mit.edu/registry/index.php/Part:BBa_F2620 BBa_F2620] || lux promoter, with luxR || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 1061bp || 3140bp || Well 6B, Plate 1 || -- | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
|| A || W || G || || 08 || [http://parts.mit.edu/registry/index.php/Part:BBa_F1610 BBa_F1610] || luxI coding region || [http://parts.mit.edu/registry/index.php/Part:pSB1AK3 pSB1AK3] || A+KR || High || 798bp || 3987bp || Well 1B, Plate 2 | || A || W || G || || 08 || [http://parts.mit.edu/registry/index.php/Part:BBa_F1610 BBa_F1610] || luxI coding region || [http://parts.mit.edu/registry/index.php/Part:pSB1AK3 pSB1AK3] || A+KR || High || 798bp || 3987bp || Well 1B, Plate 2 || -- | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
|| A || W || G || || 11 || [http://parts.mit.edu/registry/index.php/Part:BBa_E7104 BBa_E7104] || T7 GFP|| [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 826bp || 2905bp || Well 13F, Plate 2 | || A || W || G || || 11 || [http://parts.mit.edu/registry/index.php/Part:BBa_E7104 BBa_E7104] || T7 GFP|| [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 826bp || 2905bp || Well 13F, Plate 2 || -- | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
|| A || W || G || || 13 || [http://parts.mit.edu/registry/index.php/Part:BBa_B0015 BBa_B0015] || Double terminator || [http://parts.mit.edu/registry/index.php/Part:pSB1AK3 pSB1AK3] || A+KR || High || 129bp || 3316bp || Well 1B, Plate 2 | || A || W || G || || 13 || [http://parts.mit.edu/registry/index.php/Part:BBa_B0015 BBa_B0015] || Double terminator || [http://parts.mit.edu/registry/index.php/Part:pSB1AK3 pSB1AK3] || A+KR || High || 129bp || 3316bp || Well 1B, Plate 2 || -- | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
|| A || W || <font color=#990033>X</font> || || 14 || [http://parts.mit.edu/registry/index.php/Part:BBa_R0051 BBa_R0051] || λcI promoter || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 49bp || 2128bp || Well 9C, Plate 1 | || A || W || <font color=#990033>X</font> || || 14 || [http://parts.mit.edu/registry/index.php/Part:BBa_R0051 BBa_R0051] || λcI promoter || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 49bp || 2128bp || Well 9C, Plate 1 || -- | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
|| A || || || || 17 || [http://parts.mit.edu/registry/index.php/Part:BBa_I13507 BBa_I13507] || RFP || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 861bp || 2940bp || Well 16N, Plate 1 | || A || || || || 17 || [http://parts.mit.edu/registry/index.php/Part:BBa_I13507 BBa_I13507] || RFP || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 861bp || 2940bp || Well 16N, Plate 1 || -- | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
|| A || W || || || 18 || [http://parts.mit.edu/registry/index.php/Part:BBa_T9002 BBa_T9002] || GFP under lux promoter, with luxR || [http://parts.mit.edu/registry/index.php/Part:pSB1A3 pSB1A3] || AmpR || High || 1945bp || 4102bp || Well 19B, Plate 2 | || A || W || || || 18 || [http://parts.mit.edu/registry/index.php/Part:BBa_T9002 BBa_T9002] || GFP under lux promoter, with luxR || [http://parts.mit.edu/registry/index.php/Part:pSB1A3 pSB1A3] || AmpR || High || 1945bp || 4102bp || Well 19B, Plate 2 || -- | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
|| A || W || || || 19 || [http://parts.mit.edu/registry/index.php/Part:BBa_B0034 BBa_B0034] || RBS || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 12bp || 2091bp || Well 3O, Plate 1 | || A || W || || || 19 || [http://parts.mit.edu/registry/index.php/Part:BBa_B0034 BBa_B0034] || RBS || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 12bp || 2091bp || Well 3O, Plate 1 || -- | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
|| A || || || || 21 || [http://parts.mit.edu/registry/index.php/Part:BBa_J23039 BBa_J23039] || Constitutive luxI || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 860bp || 2939bp || Well 11K, Plate 3 | || A || || || || 21 || [http://parts.mit.edu/registry/index.php/Part:BBa_J23039 BBa_J23039] || Constitutive luxI || [http://parts.mit.edu/registry/index.php/Part:pSB1A2 pSB1A2] || AmpR || High || 860bp || 2939bp || Well 11K, Plate 3 || -- | ||
|- style="background:#FFCCFF" align="center" | |- style="background:#FFCCFF" align="center" | ||
|| || || || || || || || || || || || || | || || || || || || || || || || || || || | ||
|} | |} | ||
===Our Parts=== | ===Our Parts=== | ||
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|- style="background:#FFFFBB" align="center" | |- style="background:#FFFFBB" align="center" | ||
|| || || || || || BBa_I791021 || luxI coding region under | || || || || || || BBa_I791021 || luxI coding region under ptet || pSB1A2 || AmpR || High || 860bp || 2939bp || | ||
|- style="background:#FFFFBB" align="center" | |- style="background:#FFFFBB" align="center" | ||
|| || || || || || BBa_I791??? || DsRed under | || || || || || || BBa_I791??? || DsRed under ptet || pSB1A2 || AmpR || High || || || | ||
|- style="background:#FFFFBB" align="center" | |- style="background:#FFFFBB" align="center" | ||
|| || || || || || || || || || || || || | || || || || || || || || || || || || || | ||
|} | |} | ||
<font size="-2">Legend: A (Available), W (Working), G (Checked on gel) [<font color=#339900>G</font> - incorrect size; <font color=#990033>X</font> - does not appear on gel], S (Sequenced)</font> | |||
===Part Graveyard=== | ===Part Graveyard=== | ||
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|- style="background:#DDDDDD" align="center" | |- style="background:#DDDDDD" align="center" | ||
|| A || W || 10 || BBa_E1010 || RFP || pSB2K3 || 681bp || XL1, Mini || | || A || W || 10 || BBa_E1010 || RFP || pSB2K3 || 681bp || XL1, Mini || Substitute part I13507 | ||
|- style="background:#DDDDDD" align="center" | |- style="background:#DDDDDD" align="center" |
Revision as of 11:39, 15 August 2007
Constructs Overview
Cloning Table
I | ID | Part | Description | Status | Ligation Target | Use in Constructs |
02 | BBa_I13522 | ptet-GFP | XL1, Midi, Mini, BL21 | --- | Basic construct for chassis characterization | |
11 | BBa_E7104 | pT7 GFP | XL1, Midi, Mini, BL21 | --- | CBD test construct (pT7) | |
I | 18 | BBa_T9002 | ptet-luxR-plux-GFP | XL1, Midi | --- | Basic construct for chassis characterization |
22 | BBa_S03335 | pcI-GFP | Not Cloned | --- | CBD test construct (pcI) | |
05 | BBa_R0040 | ptet | XL1, Midi, Mini | DsRed | CBD final (ptet), ID AHL sender | |
BBa_I719??? | pT7 | Pending | DsRed | CBD final construct (pT7) | ||
07 | BBa_F2620 | ptet-luxR-plux | XL1, Midi, Mini | DsRed | ID final construct | |
14 | BBa_R0051 | pcI | XL1, Midi, Mini | DsRed | CBD final construct (pcI) | |
08 | BBa_F1610 | luxI | XL1, Midi, Mini | ptet | ID AHL sender final construct | |
21 | BBa_J23039 | ptet-luxI | Not Cloned | --- | ID sender construct | |
01 | BBa_E0040 | GFP | XL1, Mini | --- | Not being used anymore | |
13 | BBa_B0015 | Double terminator | XL1, Midi, Mini | --- | General part construction | |
17 | BBa_I13507 | RFP | XL1, Midi | --- | Not being used anymore | |
19 | BBa_B0034 | strong RBS | XL1, Waiting Midi | --- | General part construction | |
BBa_I719??? | acGFP | Ordered | ptet, plux, pcI | Reporter system for test constructs, Hrp | ||
BBa_I719??? | DsRed | Ordered | ptet, plux, pcI | Reporter system for ID, Hrp | ||
BBa_I719??? | HrpR-HrpS-pHrpL | Ordered | ??? | Hrp core system | ||
BBa_I719??? | HrpV | Ordered | ??? | Hrp inhibitor system | ||
Ligation Table
I | ID | Part | Description | Status | Parents | Application |
BBa_I719021 | ptet & luxI | Concerns | R0040 + F1610 | ID final sender (duplicated - J23039) | ||
BBa_I719022 | pcI & GFP | Concerns | BBa_R0051 + BBa_E0240 | CBD test construct (pcI) | ||
BBa_I719??? | pT7 & DsRed | Pending | pT7 + DsRed | CBD final construct (pT7) | ||
BBa_I719??? | ptet & DsRed | Pending | BBa_R0040+ DsRed | CBD final construct (ptet) | ||
BBa_I719??? | pcI-DsRed | Pending | BBa_R0051 + DsRed | CBD final construct (pcI) | ||
BBa_I719??? | ptet-luxR-plux-DsRed | Pending | BBa_F2620 + DsRed | ID detector final construct | ||
BBa_I719??? | hrpR-hrpS & phrpL | Pending | hrpR-hrpS + phrpL | ID detector test construct 3 and final construct 5 | ||
BBa_I719??? | Ptet-luxR-Plux & hrpR-hrpS-phrpL | Pending | BBa_F2620 + hrpR-hrpS-phrpL | ID detector test construct 3 and final construct 5 | ||
BBa_I719??? | Ptet-luxR-Plux-hrpR-hrpS-phrpL & GFP | Pending | Ptet-luxR-Plux-hrpR-hrpS-phrpL + BBa_E0240 | ID detector test construct 3 | ||
BBa_I719??? | Ptet-luxR-Plux-hrpR-hrpS-phrpL & DsRed | Pending | Ptet-luxR-Plux-hrpR-hrpS-phrpL + DsRed | ID detector final construct 5
| ||
Legend: I (Information), [Status: XL1 - cloned in XL1-Blue; Midi - midiprepped; Mini - miniprepped; BL21 - cloned in BL21(DE3)]
Parts from the Registry
A | W | G | S | ID | Name | Description | Plasmid | Resist | Copy | Size(B) | Size(T) | Location | Conc. |
A | W | G | 01 | BBa_E0040 | GFP | pSB1A2 | AmpR | High | 720bp | 2799bp | Well 5H, Plate 1 | -- | |
A | W | G | 02 | BBa_I13522 | GFP under tetracyclin promoter | pSB1A2 | AmpR | High | 937bp | 3016bp | Well 15H, Plate 2 | -- | |
A | W | X | 05 | BBa_R0040 | tetracyclin promoter | pSB1A2 | AmpR | High | 54bp | 2133bp | Well 7O, Plate 1 | -- | |
A | W | G | 07 | BBa_F2620 | lux promoter, with luxR | pSB1A2 | AmpR | High | 1061bp | 3140bp | Well 6B, Plate 1 | -- | |
A | W | G | 08 | BBa_F1610 | luxI coding region | pSB1AK3 | A+KR | High | 798bp | 3987bp | Well 1B, Plate 2 | -- | |
A | W | G | 11 | BBa_E7104 | T7 GFP | pSB1A2 | AmpR | High | 826bp | 2905bp | Well 13F, Plate 2 | -- | |
A | W | G | 13 | BBa_B0015 | Double terminator | pSB1AK3 | A+KR | High | 129bp | 3316bp | Well 1B, Plate 2 | -- | |
A | W | X | 14 | BBa_R0051 | λcI promoter | pSB1A2 | AmpR | High | 49bp | 2128bp | Well 9C, Plate 1 | -- | |
A | 17 | BBa_I13507 | RFP | pSB1A2 | AmpR | High | 861bp | 2940bp | Well 16N, Plate 1 | -- | |||
A | W | 18 | BBa_T9002 | GFP under lux promoter, with luxR | pSB1A3 | AmpR | High | 1945bp | 4102bp | Well 19B, Plate 2 | -- | ||
A | W | 19 | BBa_B0034 | RBS | pSB1A2 | AmpR | High | 12bp | 2091bp | Well 3O, Plate 1 | -- | ||
A | 21 | BBa_J23039 | Constitutive luxI | pSB1A2 | AmpR | High | 860bp | 2939bp | Well 11K, Plate 3 | -- | |||
Our Parts
A | W | G | S | ID | Name | Description | Plasmid | Resist | Copy | Size(B) | Size(P) | Stock |
BBa_I719022 | cI with GFP | pSB1A2 | AmpR | High | 933bp | 3988bp | ||||||
BBa_I791021 | luxI coding region under ptet | pSB1A2 | AmpR | High | 860bp | 2939bp | ||||||
BBa_I791??? | DsRed under ptet | pSB1A2 | AmpR | High | ||||||||
Legend: A (Available), W (Working), G (Checked on gel) [G - incorrect size; X - does not appear on gel], S (Sequenced)
Part Graveyard
A | W | ID | Name | Description | Plasmid | Size | Status | Notes |
A | 03 | BBa_J5528 | pBad-GFP | pSB2K3 | 2093bp | failed-XL1 | ara construct abandoned | |
A | 04 | BBa_J24677 | plux-GFP | pSB1A2 | 1787bp | XL1, Mini | Wrong GFP ligated | |
A | 06 | BBa_I0500 | pBad | pSB2K3 | 1210bp | XL1, Mini, Midi | ara construct abandoned | |
A | W | 08 | BBa_F1610 | luxI | pSB1AK3 | 798bp | XL1, Mini, Midi | Substitute part J23039 |
A | W | 09 | BBa_R0010 | lacI-plac | pSB1A2 | 200bp | XL1, Mini | Lac construct abandoned |
A | W | 10 | BBa_E1010 | RFP | pSB2K3 | 681bp | XL1, Mini | Substitute part I13507 |
A | 12 | BBa_S03550 | araC | pSB1AC3 | 958bp | XL1, Mini, Midi | araC construct abandoned | |
A | 15 | BBa_J07037 | cI-GFP | pSB2K3 | 912bp | XL1, Mini | No RBS present on this part | |
A | W | 16 | BBa_E0240 | GFP | pSB1A2 | 876bp | XL1, Midi | Redundant |
20 | BBa_I791020 | AraC | pSB1AC3 | 1095bp | Planned | araC construct abandoned | ||
Constructs for Cell By Date
- Construct CBD 1
- Construct CBD 2
- Entire construct is currently in Biobricks Registry: BBa_I13522 (available), in pSB1A2 (AmpR)
- pTet can be used with E.coli RNAP in vitro and maybe in veso, and it gives constitutive expression.
- Construct CBD 3
- pBAD promoter with GFP is currently available in Biobricks Registry: BBa_J5528 (available), in pSB2K3 (KanR)
- Need Ara C regulator protein in vitro and in veso. Ara C available with the RBS:BBa_S03550
- pBad can be used with E.coli RNAP in vitro, and it is inducible by arabinose.
- To be used in veso, arabinose transport proteins have to be inserted in the phospholipid bilayer of the vesicles.
- Construct CBD 4
- Entire construct is currently available in Biobricks Registry: BBa_J07037 (avaiable), in pSB2K3 (KanR)
- pcI gives constitutive expression.
- Construct CBD 5
- Construct CBD 6
- T7 promoter is in the process of cloning
- dsRed has been ordered
- Construct CBD 7
- tet promoter available in the registry: BBa_R0040
- dsRed has been ordered
- Construct CBD 8
- Construct CBD 9
- λcI regulated promoter available in the registry:BBa_R0051
- Construct CBD 10
- Parts avaiable ....
Constructs for Infector Detector
- Construct ID 1
- Construct ID 2
- Entire part is currently in Biobricks Registry: BBa_J24677 (available), in pSB1A2 (AmpR)
- Entire part minus GFP reporter system is currently in Biobricks Registry: BBa_F2620 (available), in pSB1A2 (AmpR)
- GFP reporter system is currently in Biobricks Registry: BBa_I13504 (available)
- pLux can be used with E.coli RNAP in vitro and maybe in veso, and it is repressed by LuxR but activated by AHL:LuxR complex.
- Construct ID 3
- Construct ID 4
- Construct ID 5