IGEM:IMPERIAL/2007/DNA Constructs/Con Diagrams old

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(New page: *Construct CBD 1 **Entire construct is currently available in Biobricks Registry: [http://parts.mit.edu/registry/index.php/Part:BBa_E7104 BBa_E7104] (available), in [http://parts.mit.edu/...)
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Revision as of 14:59, 30 August 2007

  • Construct CBD 1
    • Entire construct is currently available in Biobricks Registry: BBa_E7104 (available), in pSB1A2 (AmpR)
    • pT7 can be used with T7 RNAP in vitro and in veso, and it gives constitutive expression.
PT7 promoting GFP
PT7 promoting GFP

  • Construct CBD 2
    • Entire construct is currently in Biobricks Registry: BBa_I13522 (available), in pSB1A2 (AmpR)
    • pTet can be used with E.coli RNAP in vitro and maybe in veso, and it gives constitutive expression.
Ptet promoting GFP
Ptet promoting GFP

  • Construct CBD 3
    • pBAD promoter with GFP is currently available in Biobricks Registry: BBa_J5528 (available), in pSB2K3 (KanR)
    • Need Ara C regulator protein in vitro and in veso. Ara C available with the RBS:BBa_S03550
    • pBad can be used with E.coli RNAP in vitro, and it is inducible by arabinose.
    • To be used in veso, arabinose transport proteins have to be inserted in the phospholipid bilayer of the vesicles.
PBAD promoting GFP and Ptet promoting araC
PBAD promoting GFP and Ptet promoting araC

  • Construct CBD 4
    • Entire construct is currently available in Biobricks Registry: BBa_J07037 (avaiable), in pSB2K3 (KanR)
    • pcI gives constitutive expression.
PcI promoting GFP
PcI promoting GFP

PBAD promoting GFP and PcI promoting araC
PBAD promoting GFP and PcI promoting araC

  • Construct CBD 6
    • T7 promoter is in the process of cloning
    • dsRed has been ordered
pT7 promoting dsRed
pT7 promoting dsRed

  • Construct CBD 7
    • tet promoter available in the registry: BBa_R0040
    • dsRed has been ordered
Ptet promoting dsRed
Ptet promoting dsRed


  • Construct CBD 8
PBAD promoting RFP and Ptet promoting araC
PBAD promoting RFP and Ptet promoting araC


  • Construct CBD 9
    • λcI regulated promoter available in the registry:BBa_R0051
pcI promoting RFP
pcI promoting RFP


  • Construct CBD 10
    • Parts avaiable ....
PBAD promoting RFP and PcI promoting araC
PBAD promoting RFP and PcI promoting araC


Constructs for Infector Detector

  • Construct ID 1
    • Entire part minus promoter is currently in Biobricks Registry: BBa_F1610 (available), in pSB1AK3 (AmpR, KanR)
    • pTet is currently in Biobricks Registry: BBa_R0040 (available), in pSB1A2 (AmpR)
    • pTet can be used with E.coli RNAP in vitro and maybe in veso, and it gives constitutive expression.
Ptet promoting LuxI
Ptet promoting LuxI

  • Construct ID 2
    • Entire part is currently in Biobricks Registry: BBa_J24677 (available), in pSB1A2 (AmpR)
    • Entire part minus GFP reporter system is currently in Biobricks Registry: BBa_F2620 (available), in pSB1A2 (AmpR)
    • GFP reporter system is currently in Biobricks Registry: BBa_I13504 (available)
    • pLux can be used with E.coli RNAP in vitro and maybe in veso, and it is repressed by LuxR but activated by AHL:LuxR complex.
Ptet promoting LuxR, Plux promoting GFP
Ptet promoting LuxR, Plux promoting GFP

  • Construct ID 3
    • Entire part minus Hrp system is currently in Biobricks Registry: BBa_F2620(available) in pSB1A2 (AmpR)
    • pHrpL can be used with E.coli RNAP in vitro and maybe in veso, and it is inducible by HrpR:HrpS:Sigma 54 complex.
Ptet promoting LuxR, Plux promoting HrpR and HrpS and PHrpL promoting GFP
Ptet promoting LuxR, Plux promoting HrpR and HrpS and PHrpL promoting GFP

  • Construct ID 4
    • Entire part minus dsRed reporter system is currently in Biobricks Registry: BBa_F2620 (available), in pSB1A2 (AmpR)
    • dsRed has been ordered
Ptet promoting LuxI
Ptet promoting LuxI

  • Construct ID 5
    • Entire part minus the hrp system and dsRed reporter system is currently in Biobricks Registry: BBa_F2620 (available), in pSB1A2 (AmpR)
    • dsRed has been ordered
    • hrpR-hrpS-pHrpL has been ordered
Ptet promoting LuxR, Plux promoting HrpR and HrpS and PHrpL promoting lacZ
Ptet promoting LuxR, Plux promoting HrpR and HrpS and PHrpL promoting lacZ

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