IGEM:IMPERIAL/2007/Dry Lab/Log

--Anthony Lazzaro 15:23, 3 September 2007 (EDT) Welcome to the Dry Lab Log ! Here you can see what work we've been doing in Modeling & Data Analysis in a Day to Day format.

Week 9

Monday 3rd September

We have recieved some data from the Testing team and need to do the following work on it for tomorrow

$$$$ Major Problems with Excel Sheets & staggering need to have a chat to lab team in morning not much work done tonight ! $$$$$

Data to be analysed:

1.Deg GFP @ 37 Degrees

• need to normalise with respect to control
• need to extract decay coeficient

2.Deg GFP @ 20

• needs more data
• need to nomalise
• need to extract decay coeficient

3.Plux 25 Degrees

• Normalise Data wrt control
• Lag time
• Rate of GFP synthesis
• Steady State value given initial concentration

4.Ptet 20 degrees

• Turning on of Promoter
• Normalise wrt to control to get rid of increase in day 2

5.PTET @ 37 Degrees

• Get Steady State Value / Maximum Value
• Use Deg from 1. to determine K

6.Calibration cuve

• Need more data not very useful at the moment

Tuesday 4th September

• Spoke with testing team and have decided have come up with a template for importing data from notepad to excel

• Spoke with testing team about wierd results and they are currently looking into it

• Wiki design needs some work: needs to have a graphic language and make it more visual

• Started Data Analysis today :
• Degradation: We have done some simple log based calculations do determine delta
  • Deg 37
  • Deg 20
  • PTET 37
  • PTET 20
  • Plux 25