IGEM:IMPERIAL/2007/Experimental Design/Phase1/Protocol 3.2: Difference between revisions

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(New page: ==Equipments== *Fluorometer + PC *1 well plate *Plate sticker lid ==Reagents== *Pyruvate kinase *rNTPs *S30 cell extract (home made) *Reaction buffer (home made) *Commercial S30 cell extra...)
 
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**Minus Cysteine
**Minus Cysteine
**Minus Leucine
**Minus Leucine
*
*pTet DNA plasmid


==Steps==
==Steps==

Revision as of 16:35, 28 August 2007

Equipments

  • Fluorometer + PC
  • 1 well plate
  • Plate sticker lid

Reagents

  • Pyruvate kinase
  • rNTPs
  • S30 cell extract (home made)
  • Reaction buffer (home made)
  • Commercial S30 cell extract
  • Commercial Pre-incubation mix
  • Amino Acids
    • Minus Cysteine
    • Minus Leucine
  • pTet DNA plasmid

Steps

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