IGEM:IMPERIAL/2007/Experimental Design/Phase1/Protocol 3.2

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Equipments

  • Fluorometer + PC
  • 1 well plate
  • Plate sticker lid

Reagents

  • Pyruvate kinase
  • rNTPs
  • S30 cell extract (home made)
  • Reaction buffer (home made)
  • Commercial S30 cell extract
  • Commercial Pre-incubation mix
  • Amino Acids
    • Minus Cysteine
    • Minus Leucine
  • pTet DNA plasmid

Steps

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