IGEM:IMPERIAL/2007/Experimental Design/Phase1/Results 1.1
Results summary
pTet-GFP
The pTet - GFP construct has been successfully tested in vivo. The levels of fluorescence of the construct where comparable to the levels of our positive control (200x dilution of GFP) meaning that GFP was actually produced. Therefore pTet-GFP works in E.Coli.
pT7-GFP
Initial testing of the pT7 - GFP contruct in vivo failed because it was not properly induced. It will be re-tested on the 17/08/2007 after it has been induced with IPTG and left overnight to allow for a measurable GFP amount to be produced. IPTG is a T7 inducer for the E.Coli strain used (BL21).
Results update: 17/8/2007
After re-testing the pT7-GFP in vivo, no positive fluorescence reading was observed. The fluorescence levels were recorded over a range of 4 hours. The fluorescence, as seen on the graphs below, is minimal and does not change at all. Two reasons were hypothesized: The bacteria cells were in stationary phase; or that the pT7 construct is not working.
- pCI-GFP testing has been postponed due to delays in the making of the construct. Testing will be carried out as soon as the construct is ready.
- A 1 hour run (taking measurements every 2.5 min) was also conducted to test the variation on the expression levels of pTet-GFP over time.
Complete set of results and raw data . |