IGEM:IMPERIAL/2007/Experimental Design/Phase1/Results 1.2: Difference between revisions
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===<div style="font-size: 140%;"> [http://parts.mit.edu/registry/index.php/Part:BBa_T9002 pTet-LuxR-pLux-GFP] </div>=== | ===<div style="font-size: 140%;"> [http://parts.mit.edu/registry/index.php/Part:BBa_T9002 pTet-LuxR-pLux-GFP] </div>=== | ||
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The contruct was successfully tested by flooding it with a concentration of ??? nM of AHL. Since we just wanted to simply test whether the construct works or not, we did not want AHL to be a constraint and that is why a large concentration was used. Three repeated measurements were performed on three different samples and compared to a diluted concentration of GFP used as a positive control. | The contruct was successfully tested by flooding it with a concentration of ??? nM of AHL. Since we just wanted to simply test whether the construct works or not, we did not want AHL to be a constraint and that is why a large concentration was used. Three repeated measurements were performed on three different samples and compared to a diluted concentration of GFP used as a positive control. | ||
[[Image:IC2007 Experimental Design Phase 1 protocol1-2-pLux-vivo.PNG|thumb|left|400px| Results of testing pLux in vivo with a consntant AHL concentration]] | [[Image:IC2007 Experimental Design Phase 1 protocol1-2-pLux-vivo.PNG|thumb|left|400px| Results of testing pLux in vivo with a consntant AHL concentration]] |
Revision as of 03:03, 3 September 2007
Results Summary
The contruct was successfully tested by flooding it with a concentration of ??? nM of AHL. Since we just wanted to simply test whether the construct works or not, we did not want AHL to be a constraint and that is why a large concentration was used. Three repeated measurements were performed on three different samples and compared to a diluted concentration of GFP used as a positive control.