IGEM:IMPERIAL/2007/Experimental Design/Phase1/Results 1.2: Difference between revisions
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==Conlusion== | ==Conlusion== | ||
We will now test to see if pLux can express in vitro | We will now test to see if pLux can express in vitro | ||
Revision as of 07:16, 14 September 2007
In Vivo Tetsing
Aims
To Determine if the Tet-LuxR-pLux-GFP construct works in vivo after inducing at 1mM AHL concentration
Tested on Tested 17-08-2007
Results
Test: 17-08-2007
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The results show that the pLux construct works in vivo. Three repeated measurements were performed on three different samples and compared to a diluted concentration of GFP used as a positive control. On the graph on the right you can see the fluorescence reading of the pLux assembly 7 hours after it has been induced with AHL. The readings of pLux are comparable to those of the positive control, diluted GFP solution, and so we can conclude that GFP has been expressed. |
Discussion
The construct has been found to work in vivo. The results show a strong response after induction of the pLux promoter. In addition there was a lot of variability within the results, this was a problem for the pTet construct and so we will investigate the fluorometer settings to improve upon this variability
Conlusion
We will now test to see if pLux can express in vitro
