IGEM:IMPERIAL/2007/Experimental Design/Phase1/Results 1.2
Results Summary
The contruct was successfully tested by flooding it with a concentration of ??? nM of AHL. Since we just wanted to simply test whether the construct works or not, we did not want AHL to be a constraint and that is why a large concentration was used. Three repeated measurements were performed on three different samples and compared to a diluted concentration of GFP used as a positive control.
On the graph on the right you can see the fluorescence reading of the pLux assembly 7 hours after it has been induced with AHL. The readings of pLux are comparable to the diluted GFP solution and leads us to conclude that GFP has been expressed. Thus the contruct works as expected in vivo. Further tests will need to be conducted to reveal its sensitivity to AHL.
