IGEM:IMPERIAL/2007/Experimental Design/Phase1/Results 3.1: Difference between revisions
(New page: ===<div style="font-size: 140%; color: Darkblue;">Optimum counting time for the fluorometer</div>=== <hr> <br> The fluorometer we are using is a [http://www.bertholdtech.com/ww/en/pub/bioa...) |
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The fluorometer we are using is a [http://www.bertholdtech.com/ww/en/pub/bioanalytik/produkte/lb970.cfm '''Twinkle LB970'''] from | The fluorometer we are using is a [http://www.bertholdtech.com/ww/en/pub/bioanalytik/produkte/lb970.cfm '''Twinkle LB970'''] from [http://www.bertholdtech.com/ww/en/pub/zentral/firma.cfm '''Berthold Technologies''']. We noticed that by changing the counting time(window) for which the detector remains on top of each well, the variation between repeated measurements varied. We are seeking to have the less variation possible between repeats while maintaining a relatively low counting time to prevent excessive bleaching of our samples. Therefore we only examined a range of the smallest counting times possible (0.15 - 0.75 sec). | ||
To test which is the optimum time for us to use in our experiments we followed the protocol found in the previous page. The results are as follows: | To test which is the optimum time for us to use in our experiments we followed the protocol found in the previous page. For each time point examined, different samples of the same stock solution where measured repeatedly(4 times) at different windows(counting time). From there we calculated, for each window, the percentage(%) variability between the repeated measurements(4) of the same sample. The results are as follows: | ||
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The most optimum window length to be used is found to be the 0.60 sec counting time. | |||
Revision as of 08:45, 5 September 2007
Optimum counting time for the fluorometer
The fluorometer we are using is a Twinkle LB970 from Berthold Technologies. We noticed that by changing the counting time(window) for which the detector remains on top of each well, the variation between repeated measurements varied. We are seeking to have the less variation possible between repeats while maintaining a relatively low counting time to prevent excessive bleaching of our samples. Therefore we only examined a range of the smallest counting times possible (0.15 - 0.75 sec).
To test which is the optimum time for us to use in our experiments we followed the protocol found in the previous page. For each time point examined, different samples of the same stock solution where measured repeatedly(4 times) at different windows(counting time). From there we calculated, for each window, the percentage(%) variability between the repeated measurements(4) of the same sample. The results are as follows:
The most optimum window length to be used is found to be the 0.60 sec counting time.
