IGEM:IMPERIAL/2007/Experimental Design/Phase2/Results 1.1: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Peixuan Pey (talk | contribs) No edit summary |
Peixuan Pey (talk | contribs) |
||
| Line 4: | Line 4: | ||
*To determine how long the system can last (when GFP is no longer produced) at each temperature. | *To determine how long the system can last (when GFP is no longer produced) at each temperature. | ||
*To determine the maximum rate of GFP produced at each temperature range by taking fluoresence reading over a period of time ( time frame to be determined by Friday 17th August) | *To determine the maximum rate of GFP produced at each temperature range by taking fluoresence reading over a period of time ( time frame to be determined by Friday 17th August) | ||
'''Status''' | '''Status'''<br> | ||
???<br> | |||
'''Equipments''' | '''Equipments''' | ||
*Fluorometer | |||
*Fridge at 4oC | |||
*Water bath in cold room at 15oC | |||
*30oC water bath | |||
*37oC shaking incubator | |||
*50oC heating block | |||
*6 Fluorometer plates (black) | |||
*6 Sealing plate mats | |||
* | |||
* | |||
* | |||
Revision as of 16:26, 16 August 2007
Experiment
Aims
- To determine if construct expresses in vitro at temperatures of: 4oC, 15oC, 25oC, 30oC, 37oC, 50oC
- To determine how long the system can last (when GFP is no longer produced) at each temperature.
- To determine the maximum rate of GFP produced at each temperature range by taking fluoresence reading over a period of time ( time frame to be determined by Friday 17th August)
Status
???
Equipments
- Fluorometer
- Fridge at 4oC
- Water bath in cold room at 15oC
- 30oC water bath
- 37oC shaking incubator
- 50oC heating block
- 6 Fluorometer plates (black)
- 6 Sealing plate mats
