IGEM:IMPERIAL/2007/Experimental Design/Phase2/Results 1.1: Difference between revisions
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'''Steps''' | |||
Revision as of 16:42, 16 August 2007
Experiment
Aims
- To determine if construct expresses in vitro at temperatures of: 4oC, 15oC, 25oC, 30oC, 37oC, 50oC
- To determine how long the system can last (when GFP is no longer produced) at each temperature.
- To determine the maximum rate of GFP produced at each temperature range by taking fluoresence reading over a period of time ( time frame to be determined by Friday 17th August)
Status
???
Equipments
- Fluorometer
- Fridge at 4oC
- Water bath in cold room at 15oC
- 30oC water bath
- 37oC shaking incubator
- 50oC heating block
- 6 Fluorometer plates (black)
- 6 Sealing plate mats
- Gilson pipettes 1000, 200 and 20
- Eppendorf Tubes
- Plate Centrifuge
Steps
