IGEM:IMPERIAL/2007/Experimental Design/Phase2/Results 1.1: Difference between revisions

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We therefore added 4 different volumes of water in 4 wells and in each well we added the same amount of GFP (from the same stock soluton). Two wells were also used to contain just GFP(+ve) and just water(-ve) as a controls. The results are shown below:
We therefore added 4 different volumes of water in 4 wells and in each well we added the same amount of GFP (from the same stock soluton). Two wells were also used to contain just GFP(+ve) and just water(-ve) as a controls. The results are shown below:
 
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[[Image:IC2007 Experimental Design GFP Dilution in water.PNG|thumb|left|800px| Results of testing pLux in vivo with a consntant AHL concentration]]
[[Image:IC2007 Experimental Design GFP Dilution in water.PNG|thumb|left|800px| Results of testing pLux in vivo with a consntant AHL concentration]]


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On the graph on the right you can see the fluorescence reading of the pLux assembly 7 hours after it has been induced with AHL. The readings of pLux are comparable to the diluted GFP solution and leads us to conclude that GFP has been expressed. Thus the contruct works as expected in vivo. Further tests will need to be conducted to reveal its sensitivity to AHL.
It turns out that by adding more water, the reading detected actually increases. Therefore the '''fluorescence does depend on the volume''' of the substrate in which it is diluted. As you can observe on the graph, the reading of our 50ul of GFP dilution actually increases as more and more water is added. As one would expect the exact opposite to occur, the reasons behind this are still being investigated.
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Revision as of 05:01, 6 September 2007


Results Summary


GFP with variable water volumes


This purpose of this experiment was to investigate whether the same number of moles of GFP, in different water volumes gave a different fluorescence reading or not. Depending on the results, we would see whether we needed or not to maintain constant volumes when making GFP dilutions for our calibration curve.

We therefore added 4 different volumes of water in 4 wells and in each well we added the same amount of GFP (from the same stock soluton). Two wells were also used to contain just GFP(+ve) and just water(-ve) as a controls. The results are shown below:

Results of testing pLux in vivo with a consntant AHL concentration





It turns out that by adding more water, the reading detected actually increases. Therefore the fluorescence does depend on the volume of the substrate in which it is diluted. As you can observe on the graph, the reading of our 50ul of GFP dilution actually increases as more and more water is added. As one would expect the exact opposite to occur, the reasons behind this are still being investigated.

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