IGEM:IMPERIAL/2007/Notebook/2007-10-16

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(New page: ==pT7-GFPmut3b chracterisation== *The '''aim''' of today's test was to chracterise the pT7-GFPmut3b with respect to temperature *For materials and methods see the [[Imperial/Wet_Lab/Protoc...)
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==pT7-GFPmut3b chracterisation==
==pT7-GFPmut3b chracterisation==
*The '''aim''' of today's test was to chracterise the pT7-GFPmut3b with respect to temperature
*The '''aim''' of today's test was to chracterise the pT7-GFPmut3b with respect to temperature
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*For materials and methods see the [[Imperial/Wet_Lab/Protocols/Prot1.9|Protocol]]
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*For materials and methods see the [http://parts.mit.edu/igem07/index.php/Imperial/Wet_Lab/Protocols/Prot1.9|Protocol]
*The construct was tested for three temperatures: 4°C, 25°C and 37°C  
*The construct was tested for three temperatures: 4°C, 25°C and 37°C  
*The reactions were sampled every hour
*The reactions were sampled every hour
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*The [[Imperial/Wet_Lab/Results/Res1.9|Results]] showed that pT7-GFPmut3b expressed at all three temperatures and the fluorescence is the highest for greater temperatures
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*The [http://parts.mit.edu/igem07/index.php/Imperial/Wet_Lab/Results/Res1.9|Results] showed that pT7-GFPmut3b expressed at all three temperatures and the fluorescence is the highest for greater temperatures

Revision as of 21:05, 25 October 2007

pT7-GFPmut3b chracterisation

  • The aim of today's test was to chracterise the pT7-GFPmut3b with respect to temperature
  • For materials and methods see the [1]
  • The construct was tested for three temperatures: 4°C, 25°C and 37°C
  • The reactions were sampled every hour
  • The [2] showed that pT7-GFPmut3b expressed at all three temperatures and the fluorescence is the highest for greater temperatures
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