IGEM:IMPERIAL/2007/Notebook/2007-10-16: Difference between revisions
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==pT7-GFPmut3b chracterisation== | ==pT7-GFPmut3b chracterisation== | ||
*The '''aim''' of today's test was to chracterise the pT7-GFPmut3b with respect to temperature | *The '''aim''' of today's test was to chracterise the pT7-GFPmut3b with respect to temperature | ||
*For materials and methods see the [ | *For materials and methods see the [[Imperial/Wet_Lab/Protocols/Prot1.9|Protocol]] | ||
*The construct was tested for three temperatures: 4°C, 25°C and 37°C | *The construct was tested for three temperatures: 4°C, 25°C and 37°C | ||
*The reactions were sampled every hour | *The reactions were sampled every hour | ||
*The [ | *The [[Imperial/Wet_Lab/Results/Res1.9|Results]] showed that pT7-GFPmut3b expressed at all three temperatures and the fluorescence is the highest for greater temperatures | ||
Latest revision as of 18:06, 25 October 2007
pT7-GFPmut3b chracterisation
- The aim of today's test was to chracterise the pT7-GFPmut3b with respect to temperature
- For materials and methods see the Protocol
- The construct was tested for three temperatures: 4°C, 25°C and 37°C
- The reactions were sampled every hour
- The Results showed that pT7-GFPmut3b expressed at all three temperatures and the fluorescence is the highest for greater temperatures
