IGEM:IMPERIAL/2007/Notebook/2007-8-13: Difference between revisions
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**Sent to autoclave | **Sent to autoclave | ||
*Solution A for preparation of vesicles | *Solution A for preparation of vesicles | ||
==Transformation of Biobricks== | |||
#7 biobrick were transformed into XL1-Blue or BL21(DE3) | |||
#Cells were grown for 1 hour and plated | |||
#Plates were incubated at 37°C overnight | |||
*Transformed in XL1-Blue<font size=-2> | |||
**BBa_J5528 [pBad-GFP] (re-clone) | |||
**BBa_E0240 [GFP] | |||
**BBa_I13507 [RFP] | |||
**BBa_T9002 [ptet-luxR-pLux-GFP] | |||
**BBa_B0034 [RBS]</font> | |||
*Transformed in BL21(DE3)<font size=-2> | |||
**BBa_I13522 [ptet-GFP] | |||
**BBa_E7104 [T7-GFP]</font> | |||
Protocols can be found at [[IGEM:IMPERIAL/2007/Notebook/General_Protocols#Electroporation|Electroporation]] in the general protocols page | |||
Revision as of 00:12, 14 August 2007
<calendar> name=iGEM:IMPERIAL/2007/Notebook date=2007/09/15 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>
Preparation of Stock Solutions
- 1x 1 litre of 2xYT medium in Duran bottle
- 3x 1 litre of 2xYT medium in conical flask
- Sent to autoclave
- Solution A for preparation of vesicles
Transformation of Biobricks
- 7 biobrick were transformed into XL1-Blue or BL21(DE3)
- Cells were grown for 1 hour and plated
- Plates were incubated at 37°C overnight
- Transformed in XL1-Blue
- BBa_J5528 [pBad-GFP] (re-clone)
- BBa_E0240 [GFP]
- BBa_I13507 [RFP]
- BBa_T9002 [ptet-luxR-pLux-GFP]
- BBa_B0034 [RBS]
- Transformed in BL21(DE3)
- BBa_I13522 [ptet-GFP]
- BBa_E7104 [T7-GFP]
Protocols can be found at Electroporation in the general protocols page
