IGEM:IMPERIAL/2007/Notebook/2007-8-14: Difference between revisions
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#Innoculated 1 ml of culture into LB media in flask | #Innoculated 1 ml of culture into LB media in flask | ||
#Incubated overnight at 37 °C | #Incubated overnight at 37 °C | ||
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Revision as of 11:49, 15 August 2007
<calendar> name=iGEM:IMPERIAL/2007/Notebook date=2007/09/15 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>
Preparation of Stock Solutions
- 1x 1 litre of 2xYT medium in Duran bottle
- 3x 1 litre of 2xYT medium in conical flask
- Sent to autoclave
- 1x 10 ml of pre-incubation solution for preparation of cell extract
- 6x 20 ml of 1mM IPTG stock
Preparation of Cell Extract
- Picked a BL21(DE3) colony from Tet plate
- Innoculated in 100 ml of 2xYT + Tet medium
- Incubated overnight at 37 °C
Cloning of Biobricks
- Placed 5 ml of LB in a 15 ml tube
- Added appropriate antibiotics into the tube
- Picked a colony from the fresh overnight plate
- Innoculated the colony in the LB media
- Incubated for 6 hours during the day
- 2x 6 Biobricks cloned
Cloning of Biobricks (for Midiprep)
- Placed 50 ml of LB in a 250 ml conical flask
- Innoculated 1 ml of culture into LB media in flask
- Incubated overnight at 37 °C
- 2. BBa_I13422 [ptet-GFP]
- 5. BBa_R0040 [ptet]
- 7. BBa_F2620 [plux]
- 8. BBa_F1610 [luxI]
- 11. BBa_E7104 [pT7]
- 13. BBa_B0015 [stop]
- 14. BBa_R0051 [pcI]
- 16. BBa_E0240 [GFP]
- 17. BBa_I13507 [RFP]
- 18. BBa_T9002 [plux-GFP]
