IGEM:IMPERIAL/2007/Notebook/2007-8-30: Difference between revisions
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#PCR purified vector digest | #PCR purified vector digest | ||
Protocols can be found at Protocols can be found at [[IGEM:IMPERIAL/2007/Notebook/General_Protocols#Gel Extraction|Gel Extaction] in the general protocols page | |||
#Gel extract purified insert re-digest | #Gel extract purified insert re-digest | ||
#*Gel weight = 320 mg | #*Gel weight = 320 mg | ||
Protocols can be found at Protocols can be found at [[IGEM:IMPERIAL/2007/Notebook/General_Protocols#DNA Purification|DNA Purification] in the general protocols page | |||
Revision as of 12:21, 30 August 2007
<calendar> name=iGEM:IMPERIAL/2007/Notebook date=2007/09/24 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>
Construction of pT7-GFP
- Insert re-digest was run on 1% agarose gel
- 1. 30 μl insert re-digest
- 2. 5 μl uncut insert
- 3. 2 μl 1 kb DNA ladder
Protocols can be found at Protocols can be found at Electrophoresis in the general protocols page
Pictures to be put up soon
- PCR purified vector digest
Protocols can be found at Protocols can be found at [[IGEM:IMPERIAL/2007/Notebook/General_Protocols#Gel Extraction|Gel Extaction] in the general protocols page
- Gel extract purified insert re-digest
- Gel weight = 320 mg
Protocols can be found at Protocols can be found at [[IGEM:IMPERIAL/2007/Notebook/General_Protocols#DNA Purification|DNA Purification] in the general protocols page
