IGEM:IMPERIAL/2007/Notebook/2007-8-30

<calendar> name=iGEM:IMPERIAL/2007/Notebook date=2007/09/24 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>

Construction of pT7-GFP

1. Insert re-digest was run on 1% agarose gel
   • 1. 30 μl insert re-digest
   • 2. 5 μl uncut insert
   • 3. 2 μl 1 kb DNA ladder

Protocols can be found at Protocols can be found at Electrophoresis in the general protocols page

1. PCR purified vector digest
2. Gel extract purified insert re-digest
   • Gel weight = 320 mg