IGEM:IMPERIAL/2007/Notebook/2007-8-30

<calendar> name=iGEM:IMPERIAL/2007/Notebook date=2007/09/24 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>

Construction of pT7-GFP

1. Insert re-digest was run on 1% agarose gel
   • 1. 30 μl insert re-digest
   • 2. 5 μl uncut insert
   • 3. 2 μl 1 kb DNA ladder

Protocols can be found at Protocols can be found at Electrophoresis in the general protocols page
Pictures to be put up soon

1. PCR purified vector digest
2. Gel extract purified insert re-digest

Protocols can be found at Protocols can be found at DNA Extaction/Purification in the general protocols page

1. Purified vector and insert were run on 1% agarose gel
   • 1. 6 μl purified insert
   • 2. 3 μl purified vector
   • 3. 2 μl 1 kb DNA ladder

Protocols can be found at Protocols can be found at Electrophoresis in the general protocols page
Pictures to be put up soon

1. Ligated vector and insert at 14°C overnight
   • 7 μl purified insert
   • 1 μl purified vector
   • 1 μl T4 ligase
   • 1 μl T4 ligation buffer