IGEM:IMPERIAL/2007/Notebook/2007-9-12

From OpenWetWare

< IGEM:IMPERIAL | 2007(Difference between revisions)
Jump to: navigation, search
m (New page: <calendar> name=iGEM:IMPERIAL/2007/Notebook date=2007/09/12 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar> ==Expression of GFP in vitro under oil conditions== Tod...)
Current revision (09:04, 17 September 2007) (view source)
m
 
Line 11: Line 11:
==Evaporation under oil under constructs==
==Evaporation under oil under constructs==
Today we tested the evaporation under different oils. The results can be found on the following link [[IGEM:IMPERIAL/2007/Projects/Experimental_Design/Improve_Methodology/Results2]]. From these results we decided to use 30ul of paraffin oil.
Today we tested the evaporation under different oils. The results can be found on the following link [[IGEM:IMPERIAL/2007/Projects/Experimental_Design/Improve_Methodology/Results2]]. From these results we decided to use 30ul of paraffin oil.
 +
 +
==Maxiprep of Biobricks==
 +
#Maxipreped 2 Biobricks
 +
 +
<font size=-2>
 +
*13. BBa_B0015  [Stop]
 +
*25. BBa_J37032 [plux-GFP]
 +
</font>
 +
 +
Protocols can be found at [[IGEM:IMPERIAL/2007/Notebook/General_Protocols#Maxiprep|Maxiprep]] in the general protocols page

Current revision

August
SMTWTFS
1234
567891011
12131415161718
19202122232425
262728293031
September
SMTWTFS
1
2345678
9101112131415
16171819202122
23242526272829
30
October
SMTWTFS
123456
78910111213
14151617181920
21222324252627
28293031


Expression of GFP in vitro under oil conditions

Today we tested to see if pTet-GFP can express in vitro under oil conditions. We tested the construct to see if there was expression and correct folding of GFP using 30ul paraffin oil. The results are on the following link results

Evaporation under oil under constructs

Today we tested the evaporation under different oils. The results can be found on the following link IGEM:IMPERIAL/2007/Projects/Experimental_Design/Improve_Methodology/Results2. From these results we decided to use 30ul of paraffin oil.

Maxiprep of Biobricks

  1. Maxipreped 2 Biobricks

  • 13. BBa_B0015 [Stop]
  • 25. BBa_J37032 [plux-GFP]

Protocols can be found at Maxiprep in the general protocols page

Personal tools