IGEM:IMPERIAL/2007/Notebook/2007-9-14: Difference between revisions
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==Restriction Digest of Biobricks== | |||
#4 DNA samples were digested by SpeI and PstI | |||
#* Samples 5, 7, 24 | |||
#1 DNA sample wasdigested by XbaI and PstI | |||
#* Samples 23 | |||
#4 DNA samples were digested by EcoRI | |||
#* Samples 13, 25, 27b-e, T7, Kirsten's parts | |||
#4 μl of DNA was digested by 1 μl of enzymes at 37°C for 1 hour<br>Exception: Sample 23 had 6 μl DNA and 2 μl enzymes | |||
Protocols can be found at [[IGEM:IMPERIAL/2007/Notebook/General_Protocols#Restriction_Digest|Restriction Digest]] in the general protocols page | |||
==Agarose Gel Electrophoresis of Biobricks== | |||
#Checked 9 Biobricks and 13 Digests on 1% agarose gel | |||
[[Image:ICGEMS Gel 14 9.png]] | |||
Conclusions: | |||
*Part 27 is confirmed to be faulty | |||
*T7 maxi-prep was not done properly | |||
*Kirsten's parts were most likely not there... | |||
Protocols can be found at [[IGEM:IMPERIAL/2007/Notebook/General_Protocols#Electrophoresis|Electrophoresis]] in the general protocols page | |||
==Gel Purification of Biobricks== | |||
#Gel was melted and run through column | |||
#Purified DNA was eluted with ddH<sub>2</sub>O | |||
<font size=-2> | |||
*5. BBa_R0040 [ptet] (~2100 bp fragment) | |||
*7. BBa_F2620 [ptet-LuxR-pLux] (~3100 bp fragment) | |||
*23. BBa_I13504 [GFP] (~800 bp fragment) | |||
*24. BBa_R0062 [pLux] (~2100 bp fragment) | |||
</font> | |||
Protocols can be found at [[IGEM:IMPERIAL/2007/Notebook/General_Protocols#Gel_Purification|Gel Purification]] in the general protocols page |
Latest revision as of 06:36, 17 September 2007
<calendar> name=iGEM:IMPERIAL/2007/Notebook date=2007/09/12 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>
Restriction Digest of Biobricks
- 4 DNA samples were digested by SpeI and PstI
- Samples 5, 7, 24
- 1 DNA sample wasdigested by XbaI and PstI
- Samples 23
- 4 DNA samples were digested by EcoRI
- Samples 13, 25, 27b-e, T7, Kirsten's parts
- 4 μl of DNA was digested by 1 μl of enzymes at 37°C for 1 hour
Exception: Sample 23 had 6 μl DNA and 2 μl enzymes
Protocols can be found at Restriction Digest in the general protocols page
Agarose Gel Electrophoresis of Biobricks
- Checked 9 Biobricks and 13 Digests on 1% agarose gel
Conclusions:
- Part 27 is confirmed to be faulty
- T7 maxi-prep was not done properly
- Kirsten's parts were most likely not there...
Protocols can be found at Electrophoresis in the general protocols page
Gel Purification of Biobricks
- Gel was melted and run through column
- Purified DNA was eluted with ddH2O
- 5. BBa_R0040 [ptet] (~2100 bp fragment)
- 7. BBa_F2620 [ptet-LuxR-pLux] (~3100 bp fragment)
- 23. BBa_I13504 [GFP] (~800 bp fragment)
- 24. BBa_R0062 [pLux] (~2100 bp fragment)
Protocols can be found at Gel Purification in the general protocols page