IGEM:IMPERIAL/2007/Notebook/2007-9-2

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(New page: <calendar> name=iGEM:IMPERIAL/2007/Notebook date=2007/09/24 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar> ==Construction of pT7-GFP== #5 colonies grew in the lig...)
Current revision (06:59, 3 September 2007) (view source)
 
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#All 5 colonies were picked and each was grown in 5 ml of LB + Kan
#All 5 colonies were picked and each was grown in 5 ml of LB + Kan
#The 5 cultures were incubated at 37&deg;C overnight
#The 5 cultures were incubated at 37&deg;C overnight
 +
 +
==Cloning of Biobricks (for Maxiprep)==
 +
#Placed 200 ml of LB in a 500 ml conical flask
 +
#Innoculated 2 ml of culture into LB media in flask
 +
#Incubated overnight at 37 °C
 +
 +
<font size=-2>
 +
*2. BBa_I13422 [ptet-GFP]
 +
*18. BBa_T9002 [plux-GFP]
 +
</font>

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Construction of pT7-GFP

  1. 5 colonies grew in the ligation plate
  2. All 5 colonies were picked and each was grown in 5 ml of LB + Kan
  3. The 5 cultures were incubated at 37°C overnight

Cloning of Biobricks (for Maxiprep)

  1. Placed 200 ml of LB in a 500 ml conical flask
  2. Innoculated 2 ml of culture into LB media in flask
  3. Incubated overnight at 37 °C

  • 2. BBa_I13422 [ptet-GFP]
  • 18. BBa_T9002 [plux-GFP]

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