IGEM:IMPERIAL/2007/Notebook/2007-9-2
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< IGEM:IMPERIAL | 2007(Difference between revisions)
(New page: <calendar> name=iGEM:IMPERIAL/2007/Notebook date=2007/09/24 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar> ==Construction of pT7-GFP== #5 colonies grew in the lig...) |
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#All 5 colonies were picked and each was grown in 5 ml of LB + Kan | #All 5 colonies were picked and each was grown in 5 ml of LB + Kan | ||
#The 5 cultures were incubated at 37°C overnight | #The 5 cultures were incubated at 37°C overnight | ||
| + | |||
| + | ==Cloning of Biobricks (for Maxiprep)== | ||
| + | #Placed 200 ml of LB in a 500 ml conical flask | ||
| + | #Innoculated 2 ml of culture into LB media in flask | ||
| + | #Incubated overnight at 37 °C | ||
| + | |||
| + | <font size=-2> | ||
| + | *2. BBa_I13422 [ptet-GFP] | ||
| + | *18. BBa_T9002 [plux-GFP] | ||
| + | </font> | ||
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Construction of pT7-GFP
- 5 colonies grew in the ligation plate
- All 5 colonies were picked and each was grown in 5 ml of LB + Kan
- The 5 cultures were incubated at 37°C overnight
Cloning of Biobricks (for Maxiprep)
- Placed 200 ml of LB in a 500 ml conical flask
- Innoculated 2 ml of culture into LB media in flask
- Incubated overnight at 37 °C
- 2. BBa_I13422 [ptet-GFP]
- 18. BBa_T9002 [plux-GFP]
