IGEM:IMPERIAL/2007/Notebook/2007-9-2: Difference between revisions
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(New page: <calendar> name=iGEM:IMPERIAL/2007/Notebook date=2007/09/24 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar> ==Construction of pT7-GFP== #5 colonies grew in the lig...) |
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#All 5 colonies were picked and each was grown in 5 ml of LB + Kan | #All 5 colonies were picked and each was grown in 5 ml of LB + Kan | ||
#The 5 cultures were incubated at 37°C overnight | #The 5 cultures were incubated at 37°C overnight | ||
==Cloning of Biobricks (for Maxiprep)== | |||
#Placed 200 ml of LB in a 500 ml conical flask | |||
#Innoculated 2 ml of culture into LB media in flask | |||
#Incubated overnight at 37 °C | |||
<font size=-2> | |||
*2. BBa_I13422 [ptet-GFP] | |||
*18. BBa_T9002 [plux-GFP] | |||
</font> | |||
Latest revision as of 04:59, 3 September 2007
<calendar> name=iGEM:IMPERIAL/2007/Notebook date=2007/09/24 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>
Construction of pT7-GFP
- 5 colonies grew in the ligation plate
- All 5 colonies were picked and each was grown in 5 ml of LB + Kan
- The 5 cultures were incubated at 37°C overnight
Cloning of Biobricks (for Maxiprep)
- Placed 200 ml of LB in a 500 ml conical flask
- Innoculated 2 ml of culture into LB media in flask
- Incubated overnight at 37 °C
- 2. BBa_I13422 [ptet-GFP]
- 18. BBa_T9002 [plux-GFP]
