IGEM:IMPERIAL/2007/Notebook/2007-9-28: Difference between revisions
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Maira Tariq (talk | contribs) (New page: <calendar> name=iGEM:IMPERIAL/2007/Notebook date=2007/09/25 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar> ==Optimum DNA concentration== *Aim today was to get the...) |
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*Aim today was to get the optimum DNA concentration for the pTet-GFP and the pTet-lixR-pLux-GFP construct. | *Aim today was to get the optimum DNA concentration for the pTet-GFP and the pTet-lixR-pLux-GFP construct. | ||
*The constructs were tested for 0, 1, 2, 4 and 6ug of DNA. | *The constructs were tested for 0, 1, 2, 4 and 6ug of DNA. | ||
* | *The fluorescence over a period of 6 hours was recorded every hour for all the concentrations. | ||
*It was found that at 25°C pTet-lixR-pLux-GFP behaves as expected, i.e. the optimum output is for a mass between 2 and 4ug of DNA. | |||
*For pTet-GFP construct, at 37°C, the fluorecence was highest at the end of the day for 6ug of DNA. | |||
Revision as of 01:09, 1 October 2007
<calendar> name=iGEM:IMPERIAL/2007/Notebook date=2007/09/25 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>
Optimum DNA concentration
- Aim today was to get the optimum DNA concentration for the pTet-GFP and the pTet-lixR-pLux-GFP construct.
- The constructs were tested for 0, 1, 2, 4 and 6ug of DNA.
- The fluorescence over a period of 6 hours was recorded every hour for all the concentrations.
- It was found that at 25°C pTet-lixR-pLux-GFP behaves as expected, i.e. the optimum output is for a mass between 2 and 4ug of DNA.
- For pTet-GFP construct, at 37°C, the fluorecence was highest at the end of the day for 6ug of DNA.
