IGEM:IMPERIAL/2007/Notebook/2007-9-3: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Ben Yi Tew (talk | contribs) No edit summary |
|||
| Line 16: | Line 16: | ||
Protocols can be found at [[IGEM:IMPERIAL/2007/Notebook/General_Protocols#Electrophoresis|Electrophoresis]] in the general protocols page | Protocols can be found at [[IGEM:IMPERIAL/2007/Notebook/General_Protocols#Electrophoresis|Electrophoresis]] in the general protocols page | ||
#Transformed recombinant plasmid DNA into BL21 | |||
#Plated transformed cells onto LB + Kan plates | |||
#Left plates at 37°C overnight | |||
Protocols can be found at [[IGEM:IMPERIAL/2007/Notebook/General_Protocols#Transformation|Transformation]] in the general protocols page | |||
==Maxiprep of Biobricks== | ==Maxiprep of Biobricks== | ||
Revision as of 10:52, 3 September 2007
<calendar> name=iGEM:IMPERIAL/2007/Notebook date=2007/09/24 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>
Construction of pT7-GFP
- Minipreped the 5 overnight cultures
Protocols can be found at Miniprep in the general protocols page
- Digested 5μl of DNA with Nde1 and BamH1
- Checked the digestion on 1% agarose gel
Protocols can be found at Electrophoresis in the general protocols page
- Transformed recombinant plasmid DNA into BL21
- Plated transformed cells onto LB + Kan plates
- Left plates at 37°C overnight
Protocols can be found at Transformation in the general protocols page
Maxiprep of Biobricks
- Maxipreped 2 Biobricks
- 2. BBa_I13422 [ptet-GFP]
- 18. BBa_T9002 [plux-GFP]
Protocols can be found at Maxiprep in the general protocols page
