IGEM:IMPERIAL/2007/Notebook/2007-9-3
From OpenWetWare
(Difference between revisions)
(New page: <calendar> name=iGEM:IMPERIAL/2007/Notebook date=2007/09/24 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar> ==Construction of pT7-GFP== #Minipreped the 5 overnight...) |
|||
| Line 16: | Line 16: | ||
Protocols can be found at [[IGEM:IMPERIAL/2007/Notebook/General_Protocols#Electrophoresis|Electrophoresis]] in the general protocols page | Protocols can be found at [[IGEM:IMPERIAL/2007/Notebook/General_Protocols#Electrophoresis|Electrophoresis]] in the general protocols page | ||
| + | |||
| + | ==Maxiprep of Biobricks== | ||
| + | #Maxipreped 2 Biobricks | ||
| + | |||
| + | <font size=-2> | ||
| + | *2. BBa_I13422 [ptet-GFP] | ||
| + | *18. BBa_T9002 [plux-GFP] | ||
| + | </font> | ||
| + | |||
| + | Protocols can be found at [[IGEM:IMPERIAL/2007/Notebook/General_Protocols#Maxiprep|Maxiprep]] in the general protocols page | ||
Revision as of 08:01, 3 September 2007
|
|
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Construction of pT7-GFP
- Minipreped the 5 overnight cultures
Protocols can be found at Miniprep in the general protocols page
- Digested 5μl of DNA with Nde1 and BamH1
- Checked the digestion on 1% agarose gel
Protocols can be found at Electrophoresis in the general protocols page
Maxiprep of Biobricks
- Maxipreped 2 Biobricks
- 2. BBa_I13422 [ptet-GFP]
- 18. BBa_T9002 [plux-GFP]
Protocols can be found at Maxiprep in the general protocols page
