IGEM:IMPERIAL/2007/Notebook/2007-9-3

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(New page: <calendar> name=iGEM:IMPERIAL/2007/Notebook date=2007/09/24 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar> ==Construction of pT7-GFP== #Minipreped the 5 overnight...)
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Protocols can be found at [[IGEM:IMPERIAL/2007/Notebook/General_Protocols#Electrophoresis|Electrophoresis]] in the general protocols page
Protocols can be found at [[IGEM:IMPERIAL/2007/Notebook/General_Protocols#Electrophoresis|Electrophoresis]] in the general protocols page
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==Maxiprep of Biobricks==
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#Maxipreped 2 Biobricks
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<font size=-2>
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*2. BBa_I13422 [ptet-GFP]
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*18. BBa_T9002 [plux-GFP]
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</font>
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Protocols can be found at [[IGEM:IMPERIAL/2007/Notebook/General_Protocols#Maxiprep|Maxiprep]] in the general protocols page

Revision as of 08:01, 3 September 2007

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Construction of pT7-GFP

  1. Minipreped the 5 overnight cultures

Protocols can be found at Miniprep in the general protocols page

  1. Digested 5μl of DNA with Nde1 and BamH1
  2. Checked the digestion on 1% agarose gel

Protocols can be found at Electrophoresis in the general protocols page

Maxiprep of Biobricks

  1. Maxipreped 2 Biobricks

  • 2. BBa_I13422 [ptet-GFP]
  • 18. BBa_T9002 [plux-GFP]

Protocols can be found at Maxiprep in the general protocols page

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