IGEM:IMPERIAL/2007/Notebook/Lab Planning: Difference between revisions
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Ben Yi Tew (talk | contribs) (New page: This page acts as a draft for the aims and projected achievements of the web lab... equipment requests and other information might also be present on the page. ==Aims== '''Week 6:''' *Clo...) |
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Revision as of 09:31, 12 August 2007
This page acts as a draft for the aims and projected achievements of the web lab... equipment requests and other information might also be present on the page.
Aims
Week 6:
- Clone remaining bricks in XL1-Blue
- BBa_E0240 [GFP]
- BBa_I13507 [RFP]
- BBa_T9002 [ptet-luxR-pLux-GFP]
- Clone test contructs into BL21(DE3)
- BBa_I13522 [ptet-GFP]
- BBa_E7104 [T7-GFP]
- BBa_J07037 [cI-GFP]
- Midiprep bricks required for ligation
- BBa_
- BBa_
- Ligate constructs
- Make cell extracts
Outline of week:
- Monday: Cloning of plasmids, plating
- Tuesday: Growing up of colonies for mini/midiprep
- Wednesday: Mini/midiprep, ligation, checking and purification of plasmid
- Thursday: Cloning of plasmids, plating
- Friday: Growing up of colonies
Eqiupment Waiting List
A list of media to be ordered and equipment to be autoclaved
Media
- 2x YT Media
- 30x Ampcillin Plates
Autoclave Equipment
- 6x 2L conical flasks
