IGEM:IMPERIAL/2007/Notebook/Lab Planning: Difference between revisions
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**Being planned | **Being planned | ||
*Make cell extracts | *Make cell extracts | ||
'''Week 7:''' | |||
*Clone bricks in XL1-Blue<font size=-2> | |||
**BBa_J23039 [ptet-luxI] | |||
**BBa_I13504 [GFP]</font> | |||
*Clone test contructs into BL21(DE3)<font size=-2> | |||
**BBa_T9002 [plux-GFP]</font> | |||
*Ligate constructs<font size=-2> | |||
**BBa_R0051 + BBa_I13504 = BBa_I719022 [pcI + GFP]</font> | |||
Outline of week: | Outline of week: | ||
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*Tuesday: Growing up of colonies for mini/midiprep | *Tuesday: Growing up of colonies for mini/midiprep | ||
*Wednesday: Mini/midiprep | *Wednesday: Mini/midiprep | ||
*Thursday: | *Thursday: (Cloning, plating) | ||
*Friday: | *Friday: Plasmid testing, (Ligation) | ||
[Note: With an optimistic view, on cloning-ligation cycle takes 3 days.] | [Note: With an optimistic view, on cloning-ligation cycle takes 3 days.] | ||
| Line 46: | Line 56: | ||
A list of media to be ordered and equipment to be autoclaved | A list of media to be ordered and equipment to be autoclaved | ||
===Media=== | ===Media=== | ||
All media | All media has been collected | ||
===Autoclave Equipment=== | ===Autoclave Equipment=== | ||
*10x 250 ml conical flasks | *10x 250 ml conical flasks | ||
*10x 50 ml centrifuge tubes | *10x 50 ml centrifuge tubes | ||
Revision as of 03:59, 16 August 2007
This page acts as a draft for the aims and projected achievements of the web lab... equipment requests and other information might also be present on the page.
Aims
Week 6:
- Clone remaining bricks in XL1-Blue
- BBa_E0240 [GFP]
- BBa_I13507 [RFP]
- BBa_T9002 [ptet-luxR-pLux-GFP]
- BBa_B0034 [RBS]
- Clone test contructs into BL21(DE3)
- BBa_I13522 [ptet-GFP]
- BBa_E7104 [T7-GFP]
- Midiprep bricks required for ligation
- BBa_I13522 [ptet-GFP]
- BBa_R0040 [ptet]
- BBa_I0500 [pBad]
- BBa_F2620 [ptet-luxR-plux]
- BBa_F1610 [luxI]
- BBa_E7104 [T7-GFP]
- BBa_S03550 [RBS-araC]
- BBa_B0015 [Stop]
- BBa_R0051 [pcI]
- BBa_E0240 [GFP]
- BBa_I13507 [RFP]
- BBa_T9002 [plux-GFP]
- Ligate constructs
- Being planned
- Make cell extracts
Week 7:
- Clone bricks in XL1-Blue
- BBa_J23039 [ptet-luxI]
- BBa_I13504 [GFP]
- Clone test contructs into BL21(DE3)
- BBa_T9002 [plux-GFP]
- Ligate constructs
- BBa_R0051 + BBa_I13504 = BBa_I719022 [pcI + GFP]
Outline of week:
- Monday: Preparation of pre-incubation solution and YT media
- Tuesday: Growing of BL21(DE3) in YT media
- Wednesday: Harvesting of BL21(DE3)
- Thursday: Preparation of cell extracts
- Friday: Preparation of cell extracts
Cloning-ligation cycle:
- Monday: Cloning of plasmids, plating
- Tuesday: Growing up of colonies for mini/midiprep
- Wednesday: Mini/midiprep
- Thursday: (Cloning, plating)
- Friday: Plasmid testing, (Ligation)
[Note: With an optimistic view, on cloning-ligation cycle takes 3 days.]
Eqiupment Waiting List
A list of media to be ordered and equipment to be autoclaved
Media
All media has been collected
Autoclave Equipment
- 10x 250 ml conical flasks
- 10x 50 ml centrifuge tubes
