IGEM:IMPERIAL/2007/Projects: Difference between revisions

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'''[[User:Vincent Rouilly|Vincent]] 17:18, 9 July 2007 (EDT)''': A page to describe briefly the projects the team is exploring, and to link to their documentation.


=Projects@Imperial.iGEM2007=
===VesoCops - Imperial College iGEM 2007 Team===
The Imperial iGEM'07 team's choice of endeavour, and the culmination of all our brainstorming sessions.


Having endured a grueling 2 weeks brainstorming for feasible ideas, the iGEM team narrowed its selection to a few ideas, and came up with proposals for each in a hope that at least one would convince the academic staff of its worthiness as a project to pursue over 8 weeks. Our efforts paid off - and we present our prospective undertakings:
[[image:IC07_biofilm.png|thumb|right|330px|Infector Detector]]
The Imperial College iGEM 2007 team consists of ten 2nd year undergraduates from Bioengineering and Bioscience. This year, we are engineering '''VesoCops''', biological systems that report the presence of nasty bacteria. Under the Cell-Free Intelligence (CFI), we have two divisions:
* First, a surveillance team called '''Cell By Date''' that determines when food is spoilt more accurately than printed sell by dates. It exploits the thermal dependence of the rate of expression of a simple reporter system.  
* The second division consists of an undercover team - '''Infector Detector''', which detects biofilms that are antibiotic-resistant and a major source of infection in hospitals. This system makes use of Lux quorum sensing to eavesdrop on the communication between biofilm-forming bacteria.




==Part Characterization==
[[image:IC07_cellbydate.png|thumb|left|340px|Cell By Date]]
===[[IGEM:IMPERIAL/2007/Projects/In-Veso|In-Veso Gene Expression]]===
Our contribution to the synthetic biology community will be the investigation of a '''Cell-Free Chassis''', the common platform on which '''Cell By Date''' and '''Infector Detector''' will be built. The cell-free approach is particularly useful for VesoCops to operate in the medical and food industries. We believe that the characterization of this cell-free chassis will unlock fresh potential in simple constructs. Our project strategy is based on the '''Engineering Cycle''', of which we have completed specifications and design of the systems. We are starting on modelling and implementation, and we aim to test our final constructs in the new chassis. We hope that by the end of the summer, the VesoCops will be ready to start their investigations.
For more information, follow the link above to the In-Veso Gene Expression pages.
===[[IGEM:IMPERIAL/2007/Projects/Hrp System|Hrp System]]===
For more information, follow the link above to the Hrp System pages.


==Current Projects==
===[[IGEM:IMPERIAL/2007/Projects/Cell By Date|Cell By Date]]===
For more information, follow the link above to the Cell By Date pages. Project title coined by James Chappell.


===[[IGEM:IMPERIAL/2007/Biofilm Detector|Biofilm Detector]]===
<br clear="all">
For more information, follow the link above to the Biofilm Detector pages.


==Projects==
===[[IGEM:IMPERIAL/2007/Projects/Cell By Date|Cell By Date]]===
===[[IGEM:IMPERIAL/2007/Biofilm Detector|Infector Detector]]===


====[[IGEM:IMPERIAL/2007/Projects/Interesting Parts From Registry | Interesting Parts from the Registry]]====
==Chassis characterisation==
The gems we saw in our play set.
===[[IGEM:IMPERIAL/2007/Projects/In-Veso|In-Veso Gene Expression]]===


====[[IGEM:IMPERIAL/2007/Projects/Reporters | Reporters]]====
==Resources==
===[[IGEM:IMPERIAL/2007/Experimental Design| Experimental Design]]===
===[[IGEM:IMPERIAL/2007/DNA Constructs|DNA Constructs]]===
===[[IGEM:IMPERIAL/2007/Notebook/General Protocols | General Protocols]]===
Protocols being used by all projects. Examples:
* Plasmid preparation
* Plasmid extraction
* Restriction digest
* Ligation
===[[IGEM:IMPERIAL/2007/Projects/Lab Notebook|Lab Notebook]]===
A notebook of the daily activities in the lab
===[[IGEM:IMPERIAL/2007/Projects/Reporters | Reporters]]===
Any reporters that might be used for the projects
Any reporters that might be used for the projects
==Unapproved Proposals==
===[[IGEM:IMPERIAL/2007/Projects/Bac-to-Insulin|Bac-to-Insulin]]===
More info to be added.
===[[IGEM:IMPERIAL/2007/Projects/pH Buffering Machine|pH Buffering Machine]]===
For more information, follow the link above to the pH Buffering Machine page.
===Bacterial Thermostat|===
More info to be added.
===[[IGEM:IMPERIAL/2007/Projects/Biofilm Production|Biofilm Production]]===
For more information, follow the link above to the Biofilm Production page.
=====Proposal Format=====
The proposal should satisfy the following statements:
* The project is worthwhile
** It will contribute to the field of synthetic biology and the registry of parts
* The project is relevant to iGEM
** It is an engineering project, not a science project
* The project is sound
** There is real science to back up what the project needs to achieve
** A plan of action to complete the project (fit into the engineering cycle)
** A rough schedule, or at least an indication of time required for each stage of the project
* The group is able to complete the project
** Show an understanding of the lab work required
** Show that literature is available (list references)
A proposed format for the proposal is the following:
* Introduction
** What is the project? (Rough specification)
** Why is it worth pursuing?
** How is it relevant to iGEM?
* Background information and review of literature
** Summarise works relevant to the project
** Outline areas missing literature
* Project plan
** State the project objectives
** Explain how the project will be carried out to meet the objectives
** Break it down into constituent parts
** Identify possible areas of difficulty, and corresponding back-up strategies
** Identify techniques required (lab, modelling, etc.)
* Timeline
** Refer to engineering cycle
** Identify most time-consuming lab activities
** Show when each major stage should be completed by
Notes:
* Whenever possible, be quantitative
* Show understanding of the implications
* Outline the areas that are poorly understood (don't hide them)
==[[IGEM:IMPERIAL/2007/Ideas|Discarded Ideas]]==
Ideas that didn't make the cut.
==[[IGEM:IMPERIAL/2007/Projects/Lab Notebook|Lab Notebook]]==
Follow the link above to the team's lab notebook
<br>
==[[IGEM:IMPERIAL/2007/Projects/Modelling|Modelling]]==
For more information, follow the link above to the Modelling pages.

Latest revision as of 01:58, 14 August 2007


VesoCops - Imperial College iGEM 2007 Team

Infector Detector

The Imperial College iGEM 2007 team consists of ten 2nd year undergraduates from Bioengineering and Bioscience. This year, we are engineering VesoCops, biological systems that report the presence of nasty bacteria. Under the Cell-Free Intelligence (CFI), we have two divisions:

  • First, a surveillance team called Cell By Date that determines when food is spoilt more accurately than printed sell by dates. It exploits the thermal dependence of the rate of expression of a simple reporter system.
  • The second division consists of an undercover team - Infector Detector, which detects biofilms that are antibiotic-resistant and a major source of infection in hospitals. This system makes use of Lux quorum sensing to eavesdrop on the communication between biofilm-forming bacteria.


Cell By Date

Our contribution to the synthetic biology community will be the investigation of a Cell-Free Chassis, the common platform on which Cell By Date and Infector Detector will be built. The cell-free approach is particularly useful for VesoCops to operate in the medical and food industries. We believe that the characterization of this cell-free chassis will unlock fresh potential in simple constructs. Our project strategy is based on the Engineering Cycle, of which we have completed specifications and design of the systems. We are starting on modelling and implementation, and we aim to test our final constructs in the new chassis. We hope that by the end of the summer, the VesoCops will be ready to start their investigations.



Projects

Cell By Date

Infector Detector

Chassis characterisation

In-Veso Gene Expression

Resources

Experimental Design

DNA Constructs

General Protocols

Protocols being used by all projects. Examples:

  • Plasmid preparation
  • Plasmid extraction
  • Restriction digest
  • Ligation

Lab Notebook

A notebook of the daily activities in the lab

Reporters

Any reporters that might be used for the projects

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