IGEM:IMPERIAL/2007/Projects
Vincent 17:18, 9 July 2007 (EDT): A page to describe briefly the projects the team is exploring, and to link to their documentation.
Projects@Imperial.iGEM2007
The Imperial iGEM'07 team's choice of endeavour, and the culmination of all our brainstorming sessions.
Having endured a grueling 2 weeks brainstorming for feasible ideas, the iGEM team narrowed its selection to a few ideas, and came up with proposals for each in a hope that at least one would convince the academic staff of its worthiness as a project to pursue over 8 weeks. Our efforts paid off - and we present our prospective undertakings:
Part Characterization
In-Veso Gene Expression
For more information, follow the link above to the In-Veso Gene Expression pages.
Hrp System
For more information, follow the link above to the Hrp System pages.
Current Projects
Cell By Date
For more information, follow the link above to the Cell By Date pages. Project title coined by James Chappell.
Biofilm Detector
For more information, follow the link above to the Biofilm Detector pages.
DNA Constructs
For more information, follow the link above to the DNA Constructs page.
Common Resources
Interesting Parts from the Registry
The gems we saw in our play set.
General Protocols
Protocols being used by all projects. Examples:
- Plasmid preparation
- Plasmid extraction
- Restriction digest
- Ligation
Reporters
Any reporters that might be used for the projects
Lab Notebook
Follow the link above to the team's lab notebook
Equipments needed
1. LB media
2. LB+ Amplicillin plate x 30
3. LB+ Kanamycin plate x 30
4. LB+ Amp+ Kn x 15
5. 37oC shaking incubator x 1
6. 4oC freezer x 1
7. Table top centrifuge x 3
8. 4oC table top centrifuge/ cold room/ fridge
9. UV Spectrometer x 2
10. Gilson pipettes (1000, 200, 20, 10, 2) x5 each
11. Tips (yellow and blue) x 20 boxes each
12. Small tips x 5 boxes
13. Eppendorf tubes
14. Centrifuge tubes
15. Machine to check for purity of DNA (260/280)
16. 37oC water bath
17. Metal block incubator
18. Innoculation loops x 10 packets
19. 15oC water bath
20. Agarose gel equipments x 3
21. TBE x 1 litre of 50x TBE
22. Microwave oven
23. Camera
24. Blades (for cutting agarose gel) x 3
25. Band detection machine
26. EB buffer (Tris-Cl + EDTA)
27. Gloves
28. Whiteboard markers
29. Paper towels
30. 10ml pipettes x 50
31. Pipette fillers x 5
Modelling
For more information, follow the link above to the Modelling pages.
Unapproved Proposals
Bac-to-Insulin
More info to be added.
pH Buffering Machine
For more information, follow the link above to the pH Buffering Machine page.
Bacterial Thermostat|
More info to be added.
Biofilm Production
For more information, follow the link above to the Biofilm Production page.
Discarded Ideas
Ideas that didn't make the cut.