IGEM:IMPERIAL/2007/Projects/Biofilm Detector/Design

Biofilm Detection: Design

• Introduction
• Specifications
• Design
• Modelling
• Implementation
• Testing/Validation
• Notes
• References

Possible Parts

Signaling System

BBa_F1610 RBS-luxI
No promoter - we need to put one on it!

Promoter for Signaling System

pLac, if constitutive secretion
pBAD, if controlled secretion

Detection System

BBa_F2620 ptet-LuxR-pLux
Detection range: 1-1000 nM, highest sensitivity from 1-10 nM
Peak secretion: 400 GFP molecules cfu^-1 s^-1
Peak response time: 27 minutes