IGEM:IMPERIAL/2007/Projects/Biofilm Detector/Implementation/Under Construction: Difference between revisions
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==Beta-Galactosidase Assay== | |||
We will be using o-nitrophenyl-ß-galactoside as a substrate for ß-galactoside | |||
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==''Experiment 3''== | ==''Experiment 3''== | ||
'''Detector system:''' Chassis: E coli, DNA: pLux with hrp system, Reporter: B-D-Galactosidase | '''Detector system:''' Chassis: E coli, DNA: pLux with hrp system, Reporter: B-D-Galactosidase | ||
Revision as of 06:42, 3 August 2007
Beta-Galactosidase Assay
We will be using o-nitrophenyl-ß-galactoside as a substrate for ß-galactoside
Experiment 3
Detector system: Chassis: E coli, DNA: pLux with hrp system, Reporter: B-D-Galactosidase
Signaling system: AHL induced
- Measure of B-D-Galactosidase response to varying AHL concentrations:
- Same as exp A
- Measure of B-D-Galactosidase response to varying temperature conditions:
- Same as exp A
- Measure of life-span of system
- Same as exp A
Experiments in Vitro
Experiment D
Detector system:Chassis: In-vitro, DNA: pLux without hrp system, Reporter: GFP
Signaling system: AHL induced
- Measure of GFP response to varying AHL concentrations:
- Measure of GFP response to varying temperature conditions:
- Measure of life-span of system
Experiment E
Detector system:Chassis: In-vitro, DNA: pLux with hrp system, Reporter: B-D-Galactosidase
Signaling system: AHL induced
- Measure of B-D-Galactosidase response to varying AHL concentrations:
- Measure of B-D-Galactosidase response to varying temperature conditions:
- Measure of life-span of system
Experiments in veso
Experiment F
Detector system:Chassis: In-veso, DNA: pLux without hrp system, Reporter: GFP
Signaling system: AHL induced
- Measure of GFP response to varying AHL concentrations:
- Measure of GFP response to varying temperature conditions:
- Measure of life-span of system
Experiment G
Detector system:Chassis: In-veso, DNA: pLux without hrp system, Reporter: B-D-Galactosidase
Signaling system: AHL induced
- Measure of B-D-Galactosidase response to varying AHL concentrations:
- Measure of B-D-Galactosidase response to varying temperature conditions:
- Measure of life-span of system
Experiment H
Detector system:Chassis: In-veso, DNA: pLux with hrp system, Reporter: B-D-Galactosidase
Signaling system: AHL induced
- Measure of B-D-Galactosidase response to varying AHL concentrations:
- Measure of B-D-Galactosidase response to varying temperature conditions:
- Measure of life-span of system
Experiments with E. coli as a model of biofilm
Experiment I
Detector system:Chassis: In-veso, DNA: pLux with hrp system, Reporter: B-D-Galactosidase
Signaling system: AHL secreting Bacteria
- Test detector system with AHL secreting E coli, as a model of the biofilm
Experiments with biofilm
Experiment J
Detector system:Chassis: In-veso, DNA: pLux with hrp system, Reporter: B-D-Galactosidase
Signaling system: Biofilm produced in lab
- Test detector system on biofilm produced in lab
