IGEM:IMPERIAL/2007/Projects/Biofilm Detector/Modelling: Difference between revisions
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* Ptet is expressed constitutively. | * Ptet is expressed constitutively. | ||
* LuxR is present in excess of AHL | * LuxR is present in excess of AHL | ||
* Thus LuxR reaches steady-state before AHL is added. Therefore [LuxR] can be taken as constant, λ. | |||
* AHL forms a complex with LuxR, namely AHL-LuxR, which can be termed '''A'''. <br>'''A''' serves as the activator of pLuxR promoter downstream. | * AHL forms a complex with LuxR, namely AHL-LuxR, which can be termed '''A'''. <br>'''A''' serves as the activator of pLuxR promoter downstream. | ||
Revision as of 08:13, 3 August 2007
Biofilm Detection: Modelling
Diffusion coefficient of HSL in water - 4.9*10-6 Daq cm2/s
Diffusion coefficient is approximately half in biofilms
[1]
Modelling Detector for AHL
Production of AHL-LuxR Complex
Assumptions
Formation of AHL-LuxR
- Ptet is expressed constitutively.
- LuxR is present in excess of AHL
- Thus LuxR reaches steady-state before AHL is added. Therefore [LuxR] can be taken as constant, λ.
- AHL forms a complex with LuxR, namely AHL-LuxR, which can be termed A.
A serves as the activator of pLuxR promoter downstream.
