IGEM:IMPERIAL/2007/Projects/Biofilm Detector/Modelling: Difference between revisions
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====Construct to be employed==== | ====Construct to be employed==== | ||
[[image:ICGEMS_sys_Ptet_luxR_Plux_gfp.png|left|thumb|400px|Ptet promoting LuxR, Plux promoting GFP]] <br clear = "all"> | [[image:ICGEMS_sys_Ptet_luxR_Plux_gfp.png|left|thumb|400px|Ptet promoting LuxR, Plux promoting GFP]] <br clear = "all"> | ||
Modelled by 2006 Imperial Team, as part of molecular predation oscillator: [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/parts/BBa_T9002].<br> | |||
An operating/working version is quoted on the parts registry as [http://parts.mit.edu/registry/index.php/Part:BBa_T9002 T9002 - Link to MIT Parts Registry] | |||
The threshold for biofilm detection, via AHL quorum sensing molecule is approximately 0.1nM. | The threshold for biofilm detection, via AHL quorum sensing molecule is approximately 0.1nM. | ||
=====Considerations===== | |||
*Modelled deterministically; possibility for additional stochastic modelling. | |||
*Degradation term in model lacks dilution term - look into amending the model to incorporate dilution of specific molecule due to cell growth (dilution) | |||
Revision as of 08:18, 9 August 2007
Biofilm Detection: Modelling
Diffusion coefficient of HSL in water - 4.9*10-6 Daq cm2/s
Diffusion coefficient is approximately half in biofilms
[1]
Modelling Detector for AHL
Production of AHL-LuxR Complex
Assumptions
Formation of AHL-LuxR Complex
- Ptet is expressed constitutively.
- LuxR is present in excess of AHL
- Thus LuxR reaches steady-state before AHL is added. Therefore [LuxR] can be taken as constant, λ.
- AHL forms a complex with LuxR, namely AHL-LuxR, which can be termed A.
A serves as the activator of pLuxR promoter downstream.
Construct to be employed
Modelled by 2006 Imperial Team, as part of molecular predation oscillator: [2].
An operating/working version is quoted on the parts registry as T9002 - Link to MIT Parts Registry
The threshold for biofilm detection, via AHL quorum sensing molecule is approximately 0.1nM.
Considerations
- Modelled deterministically; possibility for additional stochastic modelling.
- Degradation term in model lacks dilution term - look into amending the model to incorporate dilution of specific molecule due to cell growth (dilution)
