IGEM:IMPERIAL/2007/Projects/Biofilm Detector/Notes: Difference between revisions
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<li>[[IGEM:IMPERIAL/2007/Projects/Biofilm Detector/Modelling|Modelling]]</li> | <li>[[IGEM:IMPERIAL/2007/Projects/Biofilm Detector/Modelling|Modelling]]</li> | ||
<li>[[IGEM:IMPERIAL/2007/Projects/Biofilm Detector/Implementation|Fabrication]]</li> | <li>[[IGEM:IMPERIAL/2007/Projects/Biofilm Detector/Implementation|Fabrication]]</li> | ||
<li>[[IGEM:IMPERIAL/2007/Projects/Biofilm Detector/TestingValidation|Testing/Validation]]</li> | <li>[[IGEM:IMPERIAL/2007/Projects/Biofilm Detector/TestingValidation|Testing]]</li> | ||
<li>[[IGEM:IMPERIAL/2007/Projects/Biofilm Detector/Data Analysis|Data Analysis]]</li> | |||
<li>[[IGEM:IMPERIAL/2007/Projects/Biofilm Detector/Validation|Validation]]</li> | |||
<li id="current">[[IGEM:IMPERIAL/2007/Projects/Biofilm Detector/Notes|Notes]]</li> | <li id="current">[[IGEM:IMPERIAL/2007/Projects/Biofilm Detector/Notes|Notes]]</li> | ||
<li>[[IGEM:IMPERIAL/2007/Projects/Biofilm Detector/References|References]]</li> | <li>[[IGEM:IMPERIAL/2007/Projects/Biofilm Detector/References|References]]</li> | ||
Revision as of 05:28, 11 September 2007
Infector Detector: Notes
Making Biofilm
- Freshly-grown growth-phase E. coli ZK1056 culture is added to sterile medium to a concentration of 5 × 107 cells/mL.
- Round 15 mm sterile glass coverslips are placed upright in the wells of a 24-well plate with sterile plastic stands
- Add 1 mL of E. coli solution to each well.
- The 24-well plate is immediately covered and transferred to a 30 °C incubator.
