IGEM:IMPERIAL/2007/Projects/Biofilm Detector/Notes: Difference between revisions
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*Create small amount of biofilm using ''E. coli'' | *Create small amount of biofilm using ''E. coli'' | ||
==Project Status== | |||
'''Protocols:''' | |||
*''E. coli'' testing protocols written | |||
*''in vitro'' protocols being refined | |||
'''Wet Lab:''' | |||
*Test plasmid under cloning (ready at Week 7) | |||
*Bricks for final constructs awaiting ligation | |||
'''Modelling:''' | |||
*AHL concentration on plux is currently being modelled | |||
Revision as of 05:47, 13 August 2007
Biofilm Detection: Notes
Project Goals
- Calibrate basic construct F2620 in E. coli
- Get basic construct F2620 working in vitro
- Describe and model differences of construct in vivo and in vitro
- Build final construct with β-galactosidase
- Calibrate final construct with β-galactosidase in E. coli
- Get basic construct with β-galactosidase working in vitro
- Model the construct in vivo and in vitro
- Optimize construct for biofilm detection
- Model the possible function of Hrp in the system
- Test Hrp in the system
- Create small amount of biofilm using E. coli
Project Status
Protocols:
- E. coli testing protocols written
- in vitro protocols being refined
Wet Lab:
- Test plasmid under cloning (ready at Week 7)
- Bricks for final constructs awaiting ligation
Modelling:
- AHL concentration on plux is currently being modelled
