Revision as of 07:50, 11 September 2007

Infector Detector: Notes

Freshly-grown growth-phase E. coli ZK1056 culture is added to sterile medium to a concentration of 5 × 107 cells/mL.
Round 15 mm sterile glass coverslips are placed upright in the wells of a 24-well plate with sterile plastic stands
Add 1 mL of E. coli solution to each well.
The 24-well plate is immediately covered and transferred to a 30 °C incubator.

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+==Making Biofilm==
+*Freshly-grown growth-phase E. coli ZK1056 culture is added to sterile medium to a concentration of 5 × 107 cells/mL.
+*Round 15 mm sterile glass coverslips are placed upright in the wells of a 24-well plate with sterile plastic stands
+*Add 1 mL of E. coli solution to each well.
-==Project Goals==
+*The 24-well plate is immediately covered and transferred to a 30 °C incubator.
-*Calibrate basic construct F2620 in ''E. coli''
-*Get basic construct F2620 working ''in vitro''
-*Describe and model differences of construct ''in vivo'' and ''in vitro''
-*Build final construct with β-galactosidase
-*Calibrate final construct with β-galactosidase in ''E. coli''
-*Get basic construct with β-galactosidase working ''in vitro''
-*Model the construct ''in vivo'' and ''in vitro''
-*Optimize construct for biofilm detection
-*Model the possible function of Hrp in the system
-*Test Hrp in the system
-*Create small amount of biofilm using ''E. coli''
-==Project Status==
-'''Protocols:'''
-*''E. coli'' testing protocols written
-*''in vitro'' protocols being refined
-'''Wet Lab:'''
-*Test plasmid under cloning (ready at Week 7)
-*Bricks for final constructs awaiting ligation
-'''Modelling:'''
-*AHL concentration on plux is currently being modelled