IGEM:IMPERIAL/2007/Projects/Biofilm Detector/Notes

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(Biofilm Detection: Notes)
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==Making Biofilm==
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*Freshly-grown growth-phase E. coli ZK1056 culture is added to sterile medium to a concentration of 5 × 107 cells/mL.  
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*Round 15 mm sterile glass coverslips are placed upright in the wells of a 24-well plate with sterile plastic stands
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*Add 1 mL of E. coli solution to each well.  
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==Project Goals==
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*The 24-well plate is immediately covered and transferred to a 30 °C incubator.
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*Calibrate basic construct F2620 in ''E. coli''
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*Get basic construct F2620 working ''in vitro''
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*Describe and model differences of construct ''in vivo'' and ''in vitro''
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*Build final construct with β-galactosidase
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*Calibrate final construct with β-galactosidase in ''E. coli''
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*Get basic construct with β-galactosidase working ''in vitro''
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*Model the construct ''in vivo'' and ''in vitro''
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*Optimize construct for biofilm detection
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*Model the possible function of Hrp in the system
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*Test Hrp in the system
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*Create small amount of biofilm using ''E. coli''
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==Project Status==
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'''Protocols:'''
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*''E. coli'' testing protocols written
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*''in vitro'' protocols being refined
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'''Wet Lab:'''
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*Test plasmid under cloning (ready at Week 7)
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*Bricks for final constructs awaiting ligation
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'''Modelling:'''
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*AHL concentration on plux is currently being modelled
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Revision as of 06:50, 11 September 2007

Infector Detector: Notes



Making Biofilm

  • Freshly-grown growth-phase E. coli ZK1056 culture is added to sterile medium to a concentration of 5 × 107 cells/mL.
  • Round 15 mm sterile glass coverslips are placed upright in the wells of a 24-well plate with sterile plastic stands
  • Add 1 mL of E. coli solution to each well.
  • The 24-well plate is immediately covered and transferred to a 30 °C incubator.
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