IGEM:IMPERIAL/2007/Projects/Biofilm Detector/Notes: Difference between revisions
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==Making Biofilm== | |||
*Freshly-grown growth-phase E. coli ZK1056 culture is added to sterile medium to a concentration of 5 × 107 cells/mL. | |||
*Round 15 mm sterile glass coverslips are placed upright in the wells of a 24-well plate with sterile plastic stands | |||
*Add 1 mL of E. coli solution to each well. | |||
== | *The 24-well plate is immediately covered and transferred to a 30 °C incubator. | ||
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Revision as of 04:50, 11 September 2007
Infector Detector: Notes
Making Biofilm
- Freshly-grown growth-phase E. coli ZK1056 culture is added to sterile medium to a concentration of 5 × 107 cells/mL.
- Round 15 mm sterile glass coverslips are placed upright in the wells of a 24-well plate with sterile plastic stands
- Add 1 mL of E. coli solution to each well.
- The 24-well plate is immediately covered and transferred to a 30 °C incubator.