IGEM:IMPERIAL/2007/Projects/Biofilm Detector/Notes

Infector Detector: Notes

• Introduction
• Specifications
• Design
• Modelling
• Implementation
• Testing/Validation
• Notes
• References

Making Biofilm

• Freshly-grown growth-phase E. coli ZK1056 culture is added to sterile medium to a concentration of 5 × 107 cells/mL.
• Round 15 mm sterile glass coverslips are placed upright in the wells of a 24-well plate with sterile plastic stands
• Add 1 mL of E. coli solution to each well.
• The 24-well plate is immediately covered and transferred to a 30 °C incubator.