IGEM:IMPERIAL/2007/Projects/Biofilm Detector/Specifications: Difference between revisions
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==== | ====Specification==== | ||
*Have sensitivity to AHL ~ 50nmol/µm<sup>3</sup> | *Have sensitivity to AHL ~ 50nmol/µm<sup>3</sup><cite>AHL_conc</cite> | ||
*Output a signal visible to naked eye within 3 hours | *Output a signal visible to naked eye within 3 hours | ||
*Have a lifespan of 4 days | *Have a lifespan of 4 days | ||
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<biblio> | |||
# AHL_conc pmid=11397086 | |||
</biblio> | |||
====Initial ideas==== | ====Initial ideas==== | ||
Revision as of 06:47, 26 July 2007
Biofilm Detection: Specifications
Specification
- Have sensitivity to AHL ~ 50nmol/µm3[1]
- Output a signal visible to naked eye within 3 hours
- Have a lifespan of 4 days
- Be applied through a gel spray or cream
- Work within temperatures between 20-30˚C
- Work in a pH range of 6-8
- Nilsson P, Olofsson A, Fagerlind M, Fagerström T, Rice S, Kjelleberg S, and Steinberg P. Kinetics of the AHL regulatory system in a model biofilm system: how many bacteria constitute a "quorum"?. J Mol Biol. 2001 Jun 8;309(3):631-40. DOI:10.1006/jmbi.2001.4697 |
Initial ideas
- be able to detect that it is in the presence of a biofilm on
- contact lenses
- catheters (which type?)
- surgical instruments
- kitchen utensils
- pipes
- food samples
- IUDs
- be able to adsorb to, and penetrate, the biofilm
- survive long enough in the biofilm in order to disperse it (probably using DspB)
- be able to disperse 99% of the biofilm material
- Parameters to quantify
- maximum time for biofilm dispersal
- minimum amount of biofilm to be dispersed
- environmental conditions for system to work in (temperature, pH, pressure)
- any others..?
