IGEM:IMPERIAL/2007/Projects/Biofilm Detector/Specifications

Biofilm Detection: Specifications

• Introduction
• Specifications
• Design
• Modelling
• Implementation
• Testing/Validation
• Notes
• References

Specification

• Have sensitivity to AHL ~ 50nM[1]
• Output a signal visible to naked eye within 3 hours (response time)
• Have a lifespan of 4 days
• Be applied through a gel spray or cream
• Work within temperatures between 20-30˚C
• Work in a pH range of 6-8

1. Nilsson P, Olofsson A, Fagerlind M, Fagerström T, Rice S, Kjelleberg S, and Steinberg P. Kinetics of the AHL regulatory system in a model biofilm system: how many bacteria constitute a "quorum"?. J Mol Biol. 2001 Jun 8;309(3):631-40. DOI:10.1006/jmbi.2001.4697 |