IGEM:IMPERIAL/2007/Projects/Biofilm Detector/Specifications
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Infector Detector: Specifications
Specification v.2 (Most Recent)
We have had to reconsider our original specifications due to :
- Phase 1 Manufacturing revealed that 1nM is undetectable by system therefore cannot meet minimum sensing requirement
- Further literature research revealed that fortunetly [AHL]=0.1nM doesn't seem to be correct target, it is more towards the [AHL]=50nM mark.
The infector detector system must:
- be able to detect AHL present in its surroundings and report it
- have sensitivity to AHL of at least 50nM[1]
- output a signal visible to naked eye within 3 hours (response time)
- have a reporter whose sensitivity increases with increasing [AHL]
- be applied through a gel spray or cream
- work within temperatures between 20-30˚C (as the biofilm detector is to be used within a hospital)
- work in a pH range of 6-8
- not contaminate the environment it is exposed to
- be portable and convenient to use
Specification v.1 (Out of Date)
The infector detector system must:
- be able to detect AHL present in its surroundings and report it
- have sensitivity to AHL of at least 0.1nM[1]
- output a signal visible to naked eye within 3 hours (response time)
- have a reporter whose sensitivity increases with increasing [AHL]
- be applied through a gel spray or cream
- work within temperatures between 20-30˚C (as the biofilm detector is to be used within a hospital)
- work in a pH range of 6-8
- not contaminate the environment it is exposed to
- be portable and convenient to use
- have a lifespan of 4 days
