IGEM:IMPERIAL/2007/Projects/Biofilm Detector/TestingValidation: Difference between revisions
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*Predicted | *Predicted length of experiment: 2 days dependent on availability of relevant equipment (shorter with 'Twinkle') | ||
Revision as of 03:03, 27 July 2007
Biofilm Detection: Testing/Validation
Schedule of experiments
Experiment 1 (E Coli)
Objectives
- Measure of GFP response to varying AHL concentrations
- Measure of GFP response to varying temperature conditions
- Measure of life-span of system
Details
1. Measure of GFP response to varying AHL concentrations
- Independent variable: [AHL]
- Range: 0 - 200µM
- Steps/Intervals: 10µM
- Dependent variable: GFP Fluorescence
- Response: Over period of 2 hours
- Sampling intervals:
- 5min over first 40min
- 10min thereafter
- Repetitions of experiment(n): 5
- Predicted length of experiment: 2 days dependent on availability of relevant equipment (shorter with 'Twinkle')
