IGEM:IMPERIAL/2007/Projects/Biofilm Detector/TestingValidation

Biofilm Detection: Testing/Validation

• Introduction
• Specifications
• Design
• Modelling
• Implementation
• Testing/Validation
• Notes
• References

Schedule of experiments

Experiment 1 (E Coli)

Objectives

1. Measure of GFP response to varying AHL concentrations
2. Measure of GFP response to varying temperature conditions
3. Measure of life-span of system

Details

1. Measure of GFP response to varying AHL concentrations

• Independent variable: [AHL]
  • Range: 0 - 200nM
  • Steps/Intervals: 10nM
• Dependent variable: GFP Fluorescence
  • Response: Over period of 2 hours
  • Sampling intervals:
  1. 5min over first 40min
  2. 10min thereafter
• Repetitions of experiment(n): 5
• Predicted length of experiment: 2 days dependent on availability of relevant equipment (shorter with 'Twinkle')

(Experiment is to be done at 30 °C)

2. Measure of GFP response to varying temperature conditions

• Independent variable: Temperature
  • Range: 20 - 40°C
  • Steps/Intervals: 5°C
• Dependent variable: GFP Fluorescence
  • Response: Over period of 2 hours
  • Sampling intervals:
  1. 5min over first 40min
  2. 10min thereafter
• Repetitions of experiment(n): 5
• Predicted length of experiment: 1 day ~ 5 hours

3. Measure of life-span of system

• Independent variable: Temperature
  • Range: 20 - 40°C
  • Steps/Intervals: 5°C
• Dependent variable: Rate of Fluorescence
  • Sampling intervals:
  1. Monitor once daily
• Repetitions of experiment(n): 3
• Predicted length of experiment: 1 - 5 days

(Important Requirement: Experiment should be started on a Monday)