IGEM:IMPERIAL/2007/Projects/Cell by date/Implementation: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
| Line 33: | Line 33: | ||
Protocols Required: | Protocols Required: | ||
*[[Knight:Electroporation]] | |||
*[[Endy:High-efficiency electroporation]] | |||
1. Degradation time | 1. Degradation time | ||
*[[Endy:RNA Half-life]] | |||
*λ-Reporters | |||
2. Rate of expression at constant temperature | 2. Rate of expression at constant temperature | ||
*Fluorometer Reading | |||
*Noting down colour change (photographs) | |||
3. Range of Reporters | 3. Range of Reporters | ||
*RFP | *Flurometer Reading | ||
*DsRed | **RFP | ||
**DsRed | |||
* | |||
4. Range of promoters | 4. Range of promoters | ||
Revision as of 07:37, 27 July 2007
Cell by Date: Implementation
List of Required Protocols
Preliminary Preparation
Phase 1
Preliminery Data
Protocols Required:
1. Degradation time
- Endy:RNA Half-life
- λ-Reporters
2. Rate of expression at constant temperature
- Fluorometer Reading
- Noting down colour change (photographs)
3. Range of Reporters
- Flurometer Reading
- RFP
- DsRed
4. Range of promoters
Data Analysis
Refer to modelling section
Validation of Model
1. Validate Vmax value
2. Validate transient response using best promoters/reporters
3.
