IGEM:IMPERIAL/2007/Projects/Cell by date/Implementation: Difference between revisions

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{|{| style="background:#F5FAFF; border: 1px solid #aabadd; color:Black"
{|{| style="background:#F5FAFF; border: 1px solid #aabadd; color:Black"
|- style="background:#aabadd; color:Black"
|- style="background:#aabadd; color:Black"
|| '''Type of Promoter''' ||'''Test Constructs''' || '''Status'''
|| '''Type of Promoter''' ||'''Test Constructs''' || '''''In vivo''''' || '''''In Vitro''''' || '''''In veso''''' || '''Status'''
|- style="background:#d5dcef"   
|- style="background:#d5dcef"   
| style="background:#aabadd" | Constitutive T7 Promoter  
| style="background:#aabadd" | Constitutive T7 Promoter  
|[[image:ICGEMS_dvc_PT7_gfp.png|left|PT7 promoting GFP]]  
|[[image:ICGEMS_dvc_PT7_gfp.png|left|PT7 promoting GFP]]  
|
|| By 15th Aug (Wed) || By 17th Aug (Fri) || ||
*Stock Availability: N/A
*Stock Availability: Soon
*Submission to Registry: Not Yet
*Submission to Registry: Available
 
|- style="background:#d5dcef"
|- style="background:#d5dcef"
| style="background:#aabadd" | Constitutive E.coli Promoter
| style="background:#aabadd" | Constitutive E.coli Promoter
|[[image:ICGEMS_dvc_Ptet_gfp.png|left|P<sub>tet</sub promoting GFP]]  
|[[image:ICGEMS_dvc_Ptet_gfp.png|left|P<sub>tet</sub promoting GFP]]  
|
|| By 15th Aug (Wed) || By 17th Aug (Fri) || ||
*Stock Availability: N/A
*Stock Availability: Soon
*Submission to Registry: Not Yet
*Submission to Registry: Available
 
|- style="background:#d5dcef"
|- style="background:#d5dcef"
| style="background:#aabadd" | Inducible E.coli Promoter
| style="background:#aabadd" | Inducible E.coli Promoter
|[[image:ICGEMS_dvc_PBad_gfp.png|left|PBad promoting GFP]]  
|[[image:ICGEMS_dvc_PBad_gfp.png|left|PBad promoting GFP]]  
|
|| Cancelled || || ||
*Stock Availability: N/A
*Stock Availability: N/A
*Submission to Registry: Not Yet
*Submission to Registry: N/A
 
|- style="background:#d5dcef"
|- style="background:#d5dcef"
| style="background:#aabadd" | Constitutive E.coli Promoter  
| style="background:#aabadd" | Constitutive E.coli Promoter  
|[[image:PcI_construct.PNG|left|PcI promoting GFP]]
|[[image:PcI_construct.PNG|left|PcI promoting GFP]]
|
|| In cloning (2nd round)|| || ||
*Stock Availability: N/A
*Stock Availability: N/A
*Submission to Registry: Not Yet
*Submission to Registry: Not Yet
|- style="background:#d5dcef"
|- style="background:#d5dcef"
| style="background:#aabadd" | Constitutive E.coli Promoter  
| style="background:#aabadd" | Constitutive E.coli Promoter  
|[[image:ICGEMS_dvc_PBad2_gfp.PNG‎ |left|pBad promoting GFP and pcI promoting araC]]
|[[image:ICGEMS_dvc_PBad2_gfp.PNG‎ |left|pBad promoting GFP and pcI promoting araC]]
|
|| Cancelled || || ||
*Stock Availability: N/A
*Stock Availability: N/A
*Submission to Registry: Not Yet
*Submission to Registry: N/A
 
|}
|}
<br>
<br>


==Lab Notebook==
==Lab Notebook==

Revision as of 13:58, 13 August 2007

Cell by Date: Implementation


Chassis Focus

Chassis Phase 1 Phase 2 Phase 3
E.coli X
In-Vitro X X X
In-Veso X X X


Test Constructs

Type of Promoter Test Constructs In vivo In Vitro In veso Status
Constitutive T7 Promoter
PT7 promoting GFP
PT7 promoting GFP
 By 15th Aug (Wed) By 17th Aug (Fri)
  • Stock Availability: Soon
  • Submission to Registry: Available
Constitutive E.coli Promoter
Ptet</sub promoting GFP
Ptet</sub promoting GFP
 By 15th Aug (Wed) By 17th Aug (Fri)
  • Stock Availability: Soon
  • Submission to Registry: Available
Inducible E.coli Promoter
PBad promoting GFP
PBad promoting GFP
 Cancelled
  • Stock Availability: N/A
  • Submission to Registry: N/A
Constitutive E.coli Promoter
PcI promoting GFP
PcI promoting GFP
 In cloning (2nd round)
  • Stock Availability: N/A
  • Submission to Registry: Not Yet
Constitutive E.coli Promoter
pBad promoting GFP and pcI promoting araC
pBad promoting GFP and pcI promoting araC
 Cancelled
  • Stock Availability: N/A
  • Submission to Registry: N/A


Lab Notebook

  • Week 5 (6 Aug): Building Constructs
  • Week 6 (13 Aug): Building Constructs / Phase 1
  • Week 7 (20 Aug):
  • Week 8 (27 Aug):
  • Week 9 (3 Sep):
  • Week 10 (10 Sep):


<calendar> name=iGEM:IMPERIAL/2007/Notebook date=2007/09/15 view=threemonths format=%name/%year-%month-%day weekstart=7 </calendar>

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