IGEM:IMPERIAL/2007/Projects/Cell by date/Implementation/List of Required Parts

DNA Constructs

Promoters

• T7 Promoter

BBa_J34814

Short description: To be used with T7 polymerase BBa_J34811

BBa_J34811

• pBad Promoter

BBa_I0500

pBad is an E. coli promoter that is tightly controlled by:

Inducer: arabinose

Repressor: AraC apparently acts as the repressor

One study concluded that arabinose can change the conformation of araC and prevent it from successfully binding to and repressing pBad.

RBS

• RBS (Elowitz)

BBa_B0034

Short description: This is the most efficient RBS in the registry, and sets the 1.0 efficiency standard. It is based on the Elowitz repressilator.

Reporter Gene

• RFP

• DsRed

• β-Galactosidase

• Luciferase

Terminator

• Double terminator (B0010-B0012)

BBa_B0015

Short description: Double terminator consisting of BBa_B0010 and BBa_B0012. This is the most commonly used terminator. It seems to be reliable. forward_efficiency: 0.984 reverse_efficiency: 0.295

Other Materials

• Plasmid Vactor pBAC874t

BBa_J61036

Short description:

• T7 RNApol

BBa_J34811

Short description: To be used with specal tRNA loading glutamate instead of stop codon (Part BBa_J34812).

• tRNA loading Glutamate on stop codon TTT

BBA_J34812